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Differences in steroid 5alpha-reductase iso-enzymes expression between normal and pathological human prostate tissue.

J Iehlé 1 F Radvanyi 1 D Gil Diez de Medina 1 Lh Ouafik 1 H. Gerard 1 D Chopin 1 Patrice Raynaud 1 P. Martin 1
1 laboratoire de cancerologie experimentale INSERM CJF 93-11
Abstract : We studied the expression level and cell-specific expression patterns of 5alpha-reductase (5alpha-R) types 1 and 2 iso-enzymes in human hyperplastic and malignant prostate tissue by semi-quantitative RT-PCR and in situ hybridisation analyses. In situ hybridisation established that 5alpha-R1 mRNA is preferentially expressed by epithelial cells and little expressed by stromal cells whereas 5alpha-R2 mRNA is expressed by both epithelium and stroma. Semi-quantitative RT-PCR has been performed on total RNA from different zones of normal prostate, BPH tissues and liver. We found that 5alpha-R1 and 5alpha-R2 mRNAs expression was near the same in all zones of normal prostate. In BPH tissue, 5alpha-R1 and 5alpha-R2 mRNAs expression was slightly but significantly increased, when it was compared to the levels recorded for normal prostate. In cancer samples, 5alpha-R1 mRNA expression was higher than in normal and hyperplastic prostate but the level of 5alpha-R2 mRNA was not statistically different from that observed in the different zones of normal prostate. In liver, 5alpha-R2 mRNA level was similar to that measured in BPH but 5alpha-R1 mRNA expression was ten times higher. The increase observed in 5alpha-R isoenzymes expression in BPH tissue could play an important role in the pathogenesis and/or maintenance of the disease.
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https://hal-amu.archives-ouvertes.fr/hal-01820270
Contributor : l'Houcine Ouafik <>
Submitted on : Thursday, June 21, 2018 - 2:16:45 PM
Last modification on : Tuesday, July 6, 2021 - 2:00:02 PM

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  • HAL Id : hal-01820270, version 1
  • PUBMED : 10416833

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UNIV-AMU

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J Iehlé, F Radvanyi, D Gil Diez de Medina, Lh Ouafik, H. Gerard, et al.. Differences in steroid 5alpha-reductase iso-enzymes expression between normal and pathological human prostate tissue.. Journal of Steroid Biochemistry and Molecular Biology, Elsevier, 1999, pp.189-95. ⟨hal-01820270⟩

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