Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Mutation analysis in the coding sequence of thymidine kinase 1 in breast and colorectal cancer.

Abstract : We report the first mutational study of thymidine kinase 1 (TK1) performed in human solid tumors. We sequenced cDNAs representing the complete coding region of TK1 in human breast (n=22) and colorectal (n=26) cancer. Codon 106 near the ATP binding site constantly differed (ATG --> GTG; Met --> Val) from the one deposited by Bradshaw and Deininger in the Genbank database (Accession number NM_003258). Silent polymorphisms at codon 11 (CCC --> CCT; Pro --> Pro) and codon 75 (GCG --> GCA; Ala --> Ala) were frequently detected in tumors as well as in normal tissues. In breast cancer the two polymorphisms were observed in 63.6% of the samples analyzed. No significant association could be found between polymorphisms and TK activity. In colorectal cancer the incidence of the two changes was 73.1% and 69.2%, respectively. Interestingly, one colon cancer with high cytosolic TK activity displayed two missense mutations located in and near the putative phosphorylation site by tyrosine kinase (s) (TAT --> CAT; Tyr --> His) and by cAMP-, cGMP-dependent protein kinase (TAC --> TGC; Tyr --> Cys), respectively; adjacent normal mucosa showed no mutation. This may open new avenues that imply TK1 activity in tumor cell proliferation.
Document type :
Journal articles
Complete list of metadata
Contributor : L'Houcine Ouafik Connect in order to contact the contributor
Submitted on : Tuesday, June 26, 2018 - 10:32:42 AM
Last modification on : Friday, October 22, 2021 - 3:27:02 AM


  • HAL Id : hal-01823537, version 1
  • PUBMED : 12699056



S Gilles, S. Romain, P. Casellas, L'H Ouafik, F. Fina, et al.. Mutation analysis in the coding sequence of thymidine kinase 1 in breast and colorectal cancer.. international Journal of Biological Markers, Wichtig Editore, 2003, pp.1-6. ⟨hal-01823537⟩



Record views