The complete and fully assembled genome sequence of Aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other Aeromonas species: investigation of the mobilome in environmental and pathogenic strains

Friedhelm Pfeiffer (1) , Maria-Antonia Zamora-Lagos (1) , Martin Blettinger (1) , Assa Yeroslaviz (1) , Andreas Dahl (1) , Stephan Gruber (2, 1) , Bianca Habermann (3, 1)

1 MPIB - Max-Planck-Institut für Biochemie = Max Planck Institute of Biochemistry
2 UNIL - Université de Lausanne = University of Lausanne
3 IBDM - Institut de Biologie du Développement de Marseille

Background: Due to the predominant usage of short-read sequencing to date, most bacterial genome sequences reported in the last years remain at the draft level. This precludes certain types of analyses, such as the in-depth analysis of genome plasticity. Results: Here we report the finalized genome sequence of the environmental strain Aeromonas salmonicida subsp. pectinolytica 34mel, for which only a draft genome with 253 contigs is currently available. Successful completion of the transposon-rich genome critically depended on the PacBio long read sequencing technology. Using finalized genome sequences of A. salmonicida subsp. pectinolytica and other Aeromonads, we report the detailed analysis of the transposon composition of these bacterial species. Mobilome evolution is exemplified by a complex transposon, which has shifted from pathogenicity-related to environmental-related gene content in A. salmonicida subsp. pectinolytica 34mel. Conclusion: Obtaining the complete, circular genome of A. salmonicida subsp. pectinolytica allowed us to perform an in-depth analysis of its mobilome. We demonstrate the mobilome-dependent evolution of this strain’s genetic profile from pathogenic to environmental.

Mots clés: Aeromonas; Complete circular genome; Transposon; Mobilome; PacBio; Long-read sequencing; Highprecision sequencing; Mobilome-dependent evolution; Pathogenic; Environmental

Domaines: Bactériologie; Génomique, Transcriptomique et Protéomique [q-bio.GN]

Friedhelm Pfeiffer, Maria-Antonia Zamora-Lagos, Martin Blettinger, Assa Yeroslaviz, Andreas Dahl, et al.. The complete and fully assembled genome sequence of Aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other Aeromonas species: investigation of the mobilome in environmental and pathogenic strains. BMC Genomics, 2018, 19 (1), pp.20. ⟨10.1186/s12864-017-4301-6⟩. ⟨hal-02066592⟩