HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Generation of a versatile BiFC ORFeome library for analyzing protein-protein interactions in live Drosophila

Abstract : Transcription factors achieve specificity by establishing intricate interaction networks that will change depending on the cell context. Capturing these interactions in live condition is however a challenging issue that requires sensitive and non-invasive methods. We present a set of fly lines, called 'multicolor BiFC library', which covers most of the Drosophila transcription factors for performing Bimolecular Fluorescence Complementation (BiFC). The multicolor BiFC library can be used to probe two different binary interactions simultaneously and is compatible for large-scale interaction screens. The library can also be coupled with established Drosophila genetic resources to analyze interactions in the developmentally relevant expression domain of each protein partner. We provide proof of principle experiments of these various applications, using Hox proteins in the live Drosophila embryo as a case study. Overall this novel collection of ready-to-use fly lines constitutes an unprecedented genetic toolbox for the identification and analysis of protein-protein interactions in vivo.
Complete list of metadata

Cited literature [51 references]  Display  Hide  Download

Contributor : Lionel Spinelli Connect in order to contact the contributor
Submitted on : Thursday, October 11, 2018 - 4:20:11 PM
Last modification on : Thursday, January 6, 2022 - 3:46:17 AM
Long-term archiving on: : Saturday, January 12, 2019 - 3:07:03 PM


elife-38853-v2 (1).pdf
Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Johannes Bischof, Marilyne Duffraisse, Edy Furger, Leiore Ajuria, Guillaume Giraud, et al.. Generation of a versatile BiFC ORFeome library for analyzing protein-protein interactions in live Drosophila. eLife, eLife Sciences Publication, 2018, 7, ⟨10.7554/eLife.38853⟩. ⟨hal-01882683⟩



Record views


Files downloads