Skip to Main content Skip to Navigation
Journal articles

Small membranous proteins of the TorE/NapE family, crutches for cognate respiratory systems in Proteobacteria

Abstract : In this report, we investigate small proteins involved in bacterial alternative respiratory systems that improve the enzymatic efficiency through better anchorage and multimerization of membrane components. Using the small protein TorE of the respiratory TMAO reductase system as a model, we discovered that TorE is part of a subfamily of small proteins that are present in proteobacteria in which they play a similar role for bacterial respiratory systems. We reveal by microscopy that, in Shewanella oneidensis MR1, alternative respiratory systems are evenly distributed in the membrane contrary to what has been described for Escherichia coli. Thus, the better efficiency of the respiratory systems observed in the presence of the small proteins is not due to a specific localization in the membrane, but rather to the formation of membranous complexes formed by TorE homologs with their c-type cytochrome partner protein. By an in vivo approach combining Clear Native electrophoresis and fluorescent translational fusions, we determined the 4:4 stoichiometry of the complexes. In addition, mild solubilization of the cytochrome indicates that the presence of the small protein reinforces its anchoring to the membrane. Therefore, assembly of the complex induced by this small protein improves the efficiency of the respiratory system.
Complete list of metadatas

Cited literature [75 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01890252
Contributor : Laure Azzopardi <>
Submitted on : Monday, October 8, 2018 - 2:39:55 PM
Last modification on : Thursday, October 11, 2018 - 1:18:13 AM

File

s41598-018-31851-2.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Olivier Lemaire, Pascale Infossi, Amine Ali Chaouche, Leon Espinosa, Silke Leimkuhler, et al.. Small membranous proteins of the TorE/NapE family, crutches for cognate respiratory systems in Proteobacteria. Scientific Reports, Nature Publishing Group, 2018, 8 (1-13576 (2018)), ⟨10.1038/s41598-018-31851-2⟩. ⟨hal-01890252⟩

Share

Metrics

Record views

96

Files downloads

208