AMU - Aix Marseille Université (Aix-Marseille Université
Jardins du Pharo
58 Boulevard Charles Livon
13284 Marseille cedex 7 - France)
Abstract : We report that half striatal cholinergic interneurons are dual transmitter cholinergic and GABAergic interneurons (CGINs) expressing ChAT, GAD65, Lhx7, and Lhx6 mRNAs, labeled with GAD and VGAT, generating monosynaptic dual cholinergic/GABAergic currents and an inhibitory pause response. Dopamine deprivation increases CGINs ongoing activity and abolishes GABAergic inhibition including the cortico-striatal pause because of high [Cl − ] i levels. Dopamine deprivation also dramatically increases CGINs dendritic arbors and monosynaptic interconnections probability, suggesting the formation of a dense CGINs network. The NKCC1 chloride importer antagonist bumetanide, which reduces [Cl − ] i levels, restores GABAergic inhibition, the cortico-striatal pause-rebound response, and attenuates motor effects of dopamine deprivation. Therefore, most of the striatal cholinergic excitatory drive is balanced by a concomitant powerful GABAergic inhibition that is impaired by dopamine deprivation. The attenuation by bumetanide of cardinal features of Parkinson's disease paves the way to a novel therapeutic strategy based on a restoration of low [Cl − ] i levels and GABAergic inhibition.
https://hal-amu.archives-ouvertes.fr/hal-01963841
Contributor : Fanny Pra <>
Submitted on : Friday, December 21, 2018 - 4:01:06 PM Last modification on : Thursday, March 11, 2021 - 3:16:27 AM Long-term archiving on: : Friday, March 22, 2019 - 5:25:13 PM
N. Lozovaya, S. Eftekhari, R. Cloarec, L. Gouty-Colomer, A. Dufour, et al.. GABAergic inhibition in dual-transmission cholinergic and GABAergic striatal interneurons is abolished in Parkinson disease. Nature Communications, Nature Publishing Group, 2018, 9 (1), ⟨10.1038/s41467-018-03802-y⟩. ⟨hal-01963841⟩