Skip to Main content Skip to Navigation
Journal articles

Transient microstructural brain anomalies and epileptiform discharges in mice defective for epilepsy and language-related NMDA receptor subunit gene Grin2a

Abstract : Objective: The epilepsy-aphasia spectrum (EAS) is a heterogeneous group of age-dependent childhood disorders characterized by sleep-activated discharges associated with infrequent seizures and language, cognitive, and behavioral deficits. Defects in the GRIN2A gene, encoding a subunit of glutamate-gated N-methyl-D-aspartate (NMDA) receptors, represent the most important cause of EAS identified so far. Neocortical or thalamic lesions were detected in a subset of severe EAS disorders, and more subtle anomalies were reported in patients with so-called "benign" phenotypes. However, whether brain structural alterations exist in the context of GRIN2A defects is unknown. Methods: Magnetic resonance diffusion tensor imaging (MR-DTI) was used to perform longitudinal analysis of the brain at 3 developmental timepoints in living mice genetically knocked out (KO) for Grin2a. In addition, electroencephalogra-phy (EEG) was recorded using multisite extracellular electrodes to characterize the neocortical activity in vivo. Results: Microstructural alterations were detected in the neocortex, the corpus callosum, the hippocampus, and the thalamus of Grin2a KO mice. Most MR-DTI alterations were detected at a specific developmental stage when mice were aged 30 days, but not at earlier (15 days) or later (2 months) ages. EEG analysis detected epileptiform discharges in Grin2a KO mice in the third postnatal week. Significance: Grin2a KO mice replicated several anomalies found in patients with EAS disorders. Transient structural alterations detected by MR-DTI recalled the age-dependent course of EAS disorders, which in humans start during childhood and show variable outcome at the onset of adolescence. Together with the epileptiform discharges detected in young Grin2a KO mice, our data suggested the existence of early anomalies in the maturation of the neocortical and thalamocortical systems. Whereas the possible relationship of those anomalies with sleep warrants further investigations, our data suggest that Grin2a KO mice may serve as an animal model to study the neu-ronal mechanisms of EAS disorders and to design new therapeutic strategies. K E Y W O R D S brain structure, EEG, epilepsy-aphasia, mouse model, MR-DTI Salmi and Bolbos contributed equally to the study.
Document type :
Journal articles
Complete list of metadata

Cited literature [47 references]  Display  Hide  Download
Contributor : Fanny PRA Connect in order to contact the contributor
Submitted on : Friday, December 21, 2018 - 4:30:42 PM
Last modification on : Wednesday, November 3, 2021 - 7:31:00 AM
Long-term archiving on: : Friday, March 22, 2019 - 4:47:14 PM


Transient microstructural brai...
Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Manal Salmi, Radu Bolbos, Sylvian Bauer, Marat Minlebaev, Nail Burnashev, et al.. Transient microstructural brain anomalies and epileptiform discharges in mice defective for epilepsy and language-related NMDA receptor subunit gene Grin2a. Epilepsia, Wiley, 2018, 59 (10), pp.1919-1930. ⟨10.1111/epi.14543⟩. ⟨hal-01963877⟩



Record views


Files downloads