Use of platelet-rich plasma in regenerative medicine: technical tools for correct quality control

Hajer Graiet (1) , Anna Lokchine (1) , Pauline Francois (2, 3) , Melanie Velier (2, 3) , Fanny Grimaud (4, 1) , Maxime Loyens (3) , Yael Berda-Haddad (3) , Julie Veran (5, 1) , Francoise Dignat-George (2, 3) , Florence Sabatier (2) , Jérémy Magalon (2, 1)

1 CBT 1409 - Centre d'Investigations Cliniques en Biothérapie
2 C2VN - Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research
3 LA CONCEPTION - Hôpital de la Conception [CHU - APHM]
4 AMU PHARM - Aix-Marseille Université - Faculté de pharmacie
5 CIC - Centre d'Investigation Clinique [Hôpital de la Conception - APHM]

background/aims Platelet-rich plasma (PRP) injections are used in sports medicine and have been the subject of increased clinical interest. However, there have been very few reports of the composition of initial whole blood and the final PRP product. The objective of this study was to provide technical tools to perform a correct characterisation of platelets, leucocytes and red blood cells (RBCs) from whole blood and PRP. Methods Blood and PRP were obtained from 26 healthy volunteers and prepared according to the varying parameters encountered within PRP process preparation and quantification (harvesting method, anticoagulant used, sampling method, counting method). Concentrations were measured at t=0, t=1, t=6 and t=24 hours. results Sampling of blood in Eppendorf tubes significantly decreased platelet concentration over time, whereas sampling in Microvette EDTA-coated tube kept platelet concentration stable until 24 hours. A non-significant difference was observed in platelet counts in PRP with impedance (median (IQR): 521.8 G/L (505.3–524.7)) and fluorescence (591.5 G/L (581.5–595.8)) methods. Other studied parameters did not influence platelet concentrations in blood or PRP samples. Leucocytes and RBC counts were similar whatever the anticoagulant, sampling, harvesting and counting methods used for both blood and PRP samples. Conclusions Systematic sampling of blood and PRP in EDTA-coated tubes for quality control is recommended. The use of a validated counter for PRP sample should also be taken into account.

Mots clés: Cell Therapy Laboratory; CBT- 1409; INSERM; Assistance

Domaines: Sciences du Vivant [q-bio]

Hajer Graiet, Anna Lokchine, Pauline Francois, Melanie Velier, Fanny Grimaud, et al.. Use of platelet-rich plasma in regenerative medicine: technical tools for correct quality control. BMJ Open Sport and Exercise Medicine , 2018, 4 (1), pp.e000442. ⟨10.1136/bmjsem-2018-000442⟩. ⟨hal-02060871⟩