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Aurin M. Vos, Edita Jurak, Peter de Gijsel, Robin A. Ohm, Bernard Henrissat, Luis G. Lugones, Mirjam A. Kabel, Han A. B. Wösten
Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus
www.plosone.org
www.plosone.org
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1 TL
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q
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Q
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BT
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1 TL
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q
1 j
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2.6532 0 Td
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Q
q
1 j
1 J
0 w
6.9998 0 0 6.9998 568.1763 369.411 cm
BT
/F11 1 Tf
1 TL
-0.0057 Tc
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(L)Tj
ET
Q
q
1 j
1 J
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10 0 0 10 571.9748 369.411 cm
BT
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1 TL
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(arabinofuranosidases)Tj
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[()]TJ
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1.0998 0 Td
[( )11()-4()10(\n)4()11()-9()-4()]TJ
/F11 1 Tf
3.447 0 Td
([)Tj
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(19)Tj
0 g
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2.0806 0 Td
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2.7099 0 Td
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3.1237 0 Td
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4.0705 0 Td
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(AXHd3)Tj
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[()17()12()-8()4()4()13( )4()6()-4()11()2()-9()12()12()]TJ
6.5083 0 Td
[()6()4()10()4()8()-3()7()2()22()7()-4()]TJ
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4.8132 0 Td
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2.4491 0 Td
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2.6249 0 Td
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2.2053 0 Td
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3.6567 0 Td
(collected)Tj
3.7361 0 Td
(after)Tj
2.0352 0 Td
(the)Tj
1.4684 0 Td
(2)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 368.7307 308.8629 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(nd)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 378.9921 304.3842 cm
BT
/F11 1 Tf
1 TL
-0.004 Tc
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2.1713 0 Td
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3.6793 0 Td
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3.3448 0 Td
(of)Tj
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1.0148 0 Td
()Tj
/F11 1 Tf
(-1,3-)Tj
ET
Q
q
1 j
1 J
0 w
6.9998 0 0 6.9998 520.2141 304.3842 cm
BT
/F11 1 Tf
1 TL
-0.0057 Tc
0 0 Td
(L)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 524.0125 304.3842 cm
BT
/F11 1 Tf
1 TL
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(-arabinofur-)Tj
-32.3997 -1.2982 Td
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4.0818 0 Td
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3.1181 0 Td
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2.2053 0 Td
[()]TJ
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1.0999 0 Td
[( )11()-4()10(\n)4()11()-9()-4()]TJ
/F11 1 Tf
3.4526 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
2.0749 0 Td
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2.4321 0 Td
(the)Tj
1.4684 0 Td
(AXHd3)Tj
3.3278 0 Td
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3.9288 0 Td
[()13()-2()]TJ
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2.5795 0 Td
(gene)Tj
2.0806 0 Td
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2.2053 0 Td
[()39()12()18()]TJ
-34.0551 -1.2983 Td
[()7()10()4()]TJ
1.7744 0 Td
[()12()-3()10()4()2()]TJ
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1.6951 0 Td
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4.6432 0 Td
(in)Tj
/F10 1 Tf
1.0374 0 Td
[()]TJ
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[( )11()-4()10(\n)4()11()-9()-4()]TJ
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3.4469 0 Td
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1.0261 0 Td
(assess)Tj
2.5228 0 Td
(whether)Tj
3.4753 0 Td
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1.1849 0 Td
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1.6724 0 Td
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(PIII)Tj
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1.7291 0 Td
(PIV.)Tj
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1.9842 0 Td
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3.7701 0 Td
(that)Tj
1.7801 0 Td
(AXHd3)Tj
3.3279 0 Td
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3.1181 0 Td
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0.8447 0 Td
(not)Tj
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(the)Tj
1.4683 0 Td
(bottleneck)Tj
4.3937 0 Td
(to)Tj
1.0261 0 Td
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3.583 0 Td
(compost)Tj
-30.6423 -1.3039 Td
(degradation.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 195.9874 cm
BT
/F4 1 Tf
1 TL
-0.0033 Tc
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3.959 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F17 1 Tf
-5.948 -1.4976 Td
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3.1512 0 Td
(and)Tj
1.7905 0 Td
(substrate)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F10 1 Tf
1 TL
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[()37()2()16()-9()4()1()-4()]TJ
3.6283 0 Td
[( )11()-4()10(\n)4()11()-9()-4()]TJ
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3.4526 0 Td
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1.8538 0 Td
(\(Sylvan,)Tj
3.4016 0 Td
(Netherlands\))Tj
5.4538 0 Td
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2.795 0 Td
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1.2756 0 Td
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2.931 0 Td
(agar)Tj
1.9275 0 Td
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3.6056 0 Td
(\(MEA;)Tj
2.9311 0 Td
(20)Tj
1.1678 0 Td
(gr)Tj
1.0602 0 Td
(l)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 338.6834 152.4472 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 347.074 148.0251 cm
BT
/F11 1 Tf
1 TL
-0.004 Tc
0 0 Td
(malt)Tj
2.0182 0 Td
(extract)Tj
2.9367 0 Td
([BD)Tj
1.8765 0 Td
(biosciences,)Tj
4.9549 0 Td
(Franklin)Tj
3.6624 0 Td
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2.6985 0 Td
(USA],)Tj
2.6816 0 Td
(1.5%)Tj
-35.5348 -1.3039 Td
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2.1486 0 Td
(2.1)Tj
1.3947 0 Td
(gr)Tj
1.0601 0 Td
(l)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 248.485 139.4645 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F11 1 Tf
1 TL
-0.004 Tc
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(MOPS,)Tj
3.118 0 Td
(pH)Tj
1.5081 0 Td
(7.0\).)Tj
1.9615 0 Td
(Mycelium)Tj
4.2746 0 Td
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1.3833 0 Td
(RNA)Tj
2.2621 0 Td
(isolation)Tj
3.6566 0 Td
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2.795 0 Td
(for)Tj
1.3889 0 Td
(16)Tj
1.1679 0 Td
(days)Tj
1.9956 0 Td
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2.211 0 Td
(4)Tj
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3.5319 0 Td
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2.3413 0 Td
(\(5)Tj
1.0318 0 Td
(x)Tj
0.686 0 Td
(5)Tj
0.6973 0 Td
(mm\))Tj
2.1997 0 Td
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(a)Tj
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(polycarbonate)Tj
5.8903 0 Td
(\(PC\))Tj
2.126 0 Td
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4.5637 0 Td
(\(diameter,)Tj
4.354 0 Td
(76)Tj
1.1679 0 Td
(mm;)Tj
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(0.1)Tj
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1.3946 0 Td
[()]TJ
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(m\))Tj
1.8879 0 Td
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4.7565 0 Td
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(1.4)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 225.0708 81.3543 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(3)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 228.6425 82.9984 cm
BT
/F11 1 Tf
1 TL
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(,)Tj
0.4422 0 Td
(1)Tj
0.6973 0 Td
(g)Tj
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(CaSO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 270.085 81.3543 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 273.6566 82.9984 cm
BT
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1 TL
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(,)Tj
0.4422 0 Td
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1.7347 0 Td
(50)Tj
1.1679 0 Td
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1.2869 0 Td
(demi)Tj
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(water)Tj
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(0.5)Tj
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4.0818 0 Td
(MEA.)Tj
2.5909 0 Td
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2.2847 0 Td
(3)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 239.074 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Double)Tj
3.3307 0 Td
(substit)Tj
2.8347 0 Td
(uted)Tj
2.133 0 Td
(xylan)Tj
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2.5087 0 Td
[(.)]TJ
/F0 1 Tf
(-1,3-L-arabin)Tj
6.1867 0 Td
(ofuranos)Tj
3.8197 0 Td
(idase)Tj
2.5654 0 Td
(producti)Tj
3.4866 0 Td
(on)Tj
1.311 0 Td
(by)Tj
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1.2615 0 Td
(Agaricus)Tj
4.0394 0 Td
(bisporus)Tj
/F0 1 Tf
3.9331 0 Td
(in)Tj
0.9779 0 Td
(compos)Tj
3.4442 0 Td
(t)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.02010)Tj
4.089 0 Td
(90)Tj
0 g
2.0906 0 Td
(July)Tj
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