Skip to Main content Skip to Navigation
Journal articles

Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins

Abstract : CRISPR-Cas systems are bacterial anti-viral systems, and bacterial viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs can also fine-tune the activity of CRISPR-based genome-editing tools. While Acrs are prevalent in phages capable of lying dormant in a CRISPR-carrying host, their orthologs have been observed only infrequently in virulent phages. Here we identify AcrIIA6, an Acr encoded in 33% of virulent Streptococcus thermophilus phage genomes. The X-ray structure of AcrIIA6 displays some features unique to this Acr family. We compare the activity of AcrIIA6 to those of other Acrs, including AcrIIA5 (also from S. thermophilus phages), and characterize their effectiveness against a range of CRISPR-Cas systems. Finally, we demonstrate that both Acr families from S. thermophilus phages inhibit Cas9-mediated genome editing of human cells.
Document type :
Journal articles
Complete list of metadatas

Cited literature [60 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-02094582
Contributor : Yves Bourne <>
Submitted on : Wednesday, April 10, 2019 - 7:44:01 AM
Last modification on : Tuesday, July 21, 2020 - 3:58:24 AM

File

s41467-018-05092-w.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Alexander Hynes, Geneviève Rousseau, Daniel Agudelo, Adeline Goulet, Béatrice Amigues, et al.. Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.2919. ⟨10.1038/s41467-018-05092-w⟩. ⟨hal-02094582⟩

Share

Metrics

Record views

161

Files downloads

316