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Journal Articles Nature Biotechnology Year : 2018

Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection

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Elodie Drula
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William Kelly
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Nikola Palevich
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Peter Janssen
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Ron Ronimus
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Samantha Noel
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Priya Soni
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Kerri Reilly
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Todd Atherly
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Cherie Ziemer
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Andre-Denis Wright
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Suzanne Ishaq
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Michael Cotta
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Stephanie Thompson
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Katie Crosley
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Nest Mckain
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R John Wallace
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Robert Forster
Robert Gruninger
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Tim Mcallister
Rosalind Gilbert
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Diane Ouwerkerk
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Athol Klieve
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Rafat Al Jassim
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Stuart Denman
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Chris Mcsweeney
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Carly Rosewarne
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Satoshi Koike
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Yasuo Kobayashi
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Makoto Mitsumori
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Takumi Shinkai
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Silvio Cravero
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María Cerón Cucchi
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Abstract

Productivity of ruminant livestock depends on the rumen microbiota, which ferment indigestible plant polysaccharides into nutrients used for growth. Understanding the functions carried out by the rumen microbiota is important for reducing greenhouse gas production by ruminants and for developing biofuels from lignocellulose. We present 410 cultured bacteria and archaea, together with their reference genomes, representing every cultivated rumen-associated archaeal and bacterial family. We evaluate polysaccharide degradation, short-chain fatty acid production and methanogenesis pathways, and assign specific taxa to functions. A total of 336 organisms were present in available rumen metagenomic data sets, and 134 were present in human gut microbiome data sets. Comparison with the human microbiome revealed rumen-specific enrichment for genes encoding de novo synthesis of vitamin B12, ongoing evolution by gene loss and potential vertical inheritance of the rumen microbiome based on underrepresentation of markers of environmental stress. We estimate that our Hungate genome resource represents ∼75% of the genus-level bacterial and archaeal taxa present in the rumen.
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Dates and versions

hal-02094598 , version 1 (09-04-2019)

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Attribution - CC BY 4.0

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Rekha Seshadri, Sinead Leahy, Graeme Attwood, Koon Hoong Teh, Suzanne Lambie, et al.. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnology, 2018, 36 (4), pp.359-367. ⟨10.1038/nbt.4110⟩. ⟨hal-02094598⟩
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