A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).

Sean Ma , Yolanda Chen , Martin Ma , Joshua Benoit , Anita Bhandari , Julia Bowsher , Kristian Brevik , Kaat Cappelle , Mei-Ju Li , Anna Childers , Christopher Li , Olivier Christiaens , Justin Ma , Elise Didion , Elena Elpidina , Patamarerk Engsontia , Markus Ma , Inmaculada García-Robles , Richard Gibbs (1) , Chandan Goswami , Alessandro Grapputo (2) , Kristina Gruden (3) , Marcin Ma , Bernard Ma , Emily Jennings , Jeffery Jones , Megha Kalsi , Sher Afzal Khan (4) , Abhishek Kumar (5) , Fei Li (6) , Vincent Ma , Xingzhou Ma , Alex Martynov , Nicholas Miller , Robert Mitchell , Monica Muñoz-Torres (7) , Anna Muszewska , Brenda Oppert , Subba Reddy Palli , Kristen Panfilio , Yannick Pauchet (8) , Lindsey Perkin , Marko Petek , Monica Poelchau (9) , Eric Record (10) , Joseph Rinehart , Hugh Robertson , Andrew Rosendale , Victor Ruiz-Arroyo , Guy Smagghe (11) , Zsofia Szendrei , Gregg W.C Thomas (12) , Alex Torson , Iris Vargas Jentzsch , Matthew Weirauch , Ashley Yates , George Yocum , June-Sun Yoon , Stephen Richards (13) , Sean Schoville , Martin Andersson (14) , Mei-Ju Chen , Christopher Childers , Justin Clements , Markus Friedrich , Marcin Grynberg , Bernard Henrissat (15) , Vincent Lombard (15) , Alexander Ma , Lindsey Li , Marko Ma , Robert Robertson , Gregg W.C. Thomas , Matthew Ma , Richard Richards

1 HGSC - Human Genome Sequencing Center [Houston]
2 CoE - Centre of Excellence in Biological interactions
3 National Institute of Biology
4 Max Planck Institute for Chemical Ecology
5 IP - Institut Pascal
6 EPFL - Ecole Polytechnique Fédérale de Lausanne
7 LBNL - Lawrence Berkeley National Laboratory [Berkeley]
8 Department of Entomology
9 Department of Biology
10 BBF - Biodiversité et Biotechnologie Fongiques
11 Faculty of Bioscience Engineering [Ghent]
12 Indiana University [Bloomington]
13 Human Genome Sequencing Center
14 Institute of Modern Physics
15 AFMB - Architecture et fonction des macromolécules biologiques

The Colorado potato beetle is one of the most challenging agricultural pests to manage. It has shown a spectacular ability to adapt to a variety of solanaceaeous plants and variable climates during its global invasion, and, notably, to rapidly evolve insecticide resistance. To examine evidence of rapid evolutionary change, and to understand the genetic basis of herbivory and insecticide resistance, we tested for structural and functional genomic changes relative to other arthropod species using genome sequencing, transcriptomics, and community annotation. Two factors that might facilitate rapid evolutionary change include transposable elements, which comprise at least 17% of the genome and are rapidly evolving compared to other Coleoptera, and high levels of nucleotide diversity in rapidly growing pest populations. Adaptations to plant feeding are evident in gene expansions and differential expression of digestive enzymes in gut tissues, as well as expansions of gustatory receptors for bitter tasting. Surprisingly, the suite of genes involved in insecticide resistance is similar to other beetles. Finally, duplications in the RNAi pathway might explain why Leptinotarsa decemlineata has high sensitivity to dsRNA. The L. decemlineata genome provides opportunities to investigate a broad range of phenotypes and to develop sustainable methods to control this widely successful pest.

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]

Sean Ma, Yolanda Chen, Martin Ma, Joshua Benoit, Anita Bhandari, et al.. A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Scientific Reports, 2018, 8 (1), pp.1931. ⟨10.1038/s41598-018-20154-1⟩. ⟨hal-02094614⟩