Lat(Y136F) knock-in mouse model for human IgG4-related disease

Kazunori Yamada (1) , Masahiko Zuka (2) , Kiyoaki Ito (2) , Keishi Mizuguchi (2) , Yasushi Kakuchi (2) , Tamehito Onoe (2) , Yasunori Suzuki (3) , Masakazu Yamagishi (2) , Shozo Izui (4) , Marie Malissen (5) , Bernard Malissen (5) , Mitsuhiro Kawano (2)

1 Department of Molecular Biosciences [Lawrence]
2 KU - Kanazawa University
3 HIP/JAIST - Human Information Processing Laboratory - Japanese Advances Institute of Science and Technology [Ishikawa]
4 UNIGE - Université de Genève = University of Geneva
5 CIML - Centre d'Immunologie de Marseille - Luminy

Background The adaptor protein Linker for activation of T cell (LAT) is a key signaling hub used by the T cell antigen receptor. Mutant mice expressing loss-of-function mutations affecting LAT and including a mutation in which tyrosine 136 is replaced by a phenylalanine (Lat(Y136F)) develop lymphoproliferative disorder involving T helper type 2 effector cells capable of triggering a massive polyclonal B cell activation that leads to hypergammaglobulinemia G1 and E and to non-resolving inflammation and autoimmunity. The purpose of this study was to evaluate whether the phenotypes of Lat(Y136F) knock-in mice resemble the immunohistopathological features of immunoglobulin G4-related disease (IgG4-RD). Methods Lat(Y136F) knock-in mice were sacrificed at 4-20 weeks of age, and pancreas, kidney, salivary gland and lung were obtained. All organs were stained with hematoxylin-eosin and with Azan for estimation of collagen in fibrosis, and the severity scores of inflammation and fibrosis were evaluated. Immunostainings were performed to analyze the types of infiltrating cells. In addition, the effects of corticosteroid treatment on the development of tissue lesions and serum levels of IgG1 were assessed. Results Tissue lesions characterized by inflammatory mononuclear cell infiltration and fibrosis were detected in pancreas, kidney, and salivary gland starting from 6 weeks of age. Immunostainings showed pronounced infiltration of plasma cells, CD4-positive T cells, and macrophages. Infiltrating plasma cells predominantly expressed IgG1. The extent of inflammation in pancreas and salivary glands was markedly reduced by corticosteroid treatment. Conclusions Lat(Y136F) knock-in mice displayed increased production of Th2-type IgG1 (a homologue of human IgG4) and developed multiple organ tissue lesions reminiscent of those seen in patients with IgG4-RD. Moreover, the development of these tissue lesions was highly sensitive to corticosteroid treatment like in IgG4-RD. For these reasons we consider the Lat(Y136F) knock-in mouse strain to represent a promising model for human IgG4-RD.

Mots clés: Kidneys; Pancreas; Salivary glands; Mouse models; Inflammation; Fibrosis; Lesions; Plasma cells

Domaines: Immunologie

Kazunori Yamada, Masahiko Zuka, Kiyoaki Ito, Keishi Mizuguchi, Yasushi Kakuchi, et al.. Lat(Y136F) knock-in mouse model for human IgG4-related disease. PLoS ONE, 2018, 13 (6), pp.e0198417. ⟨10.1371/journal.pone.0198417⟩. ⟨hal-02117995⟩