Skip to Main content Skip to Navigation
Journal articles

Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae)

Abstract : Premise Gladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species. Methods and Results We utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite‐enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62–3.08 alleles per population (effective alleles: 1.58–2.08) that indicated moderate to high genetic diversity values (0.28–0.44) in three pilot populations. Cross‐species amplification was less effective in G. imbricatus and G. tenuis. Conclusions The primers reported here can be used for the population genetic characterization of G. palustris. They will help us to better understand the conservation genetics of this highly endangered species.
Complete list of metadatas

https://hal-amu.archives-ouvertes.fr/hal-02129204
Contributor : Emese Meglécz <>
Submitted on : Tuesday, May 14, 2019 - 4:52:46 PM
Last modification on : Tuesday, February 11, 2020 - 10:18:04 AM

Links full text

Identifiers

Citation

Tamás Malkócs, Shyryn Almerekova, Judit Bereczki, Judit Cservenka, Emese Meglécz, et al.. Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae). Applications in Plant Sciences, Wiley, 2019, pp.e01245. ⟨10.1002/aps3.1245⟩. ⟨hal-02129204⟩

Share

Metrics

Record views

72