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Interleukin-22-deficiency and microbiota contribute to the exacerbation of Toxoplasma gondii-induced intestinal inflammation

Abstract : Upon oral infection with Toxoplasma gondii cysts (76 K strain) tachyzoites are released into the intestinal lumen and cross the epithelial barrier causing damage and acute intestinal inflammation in C57BL/6 (B6) mice. Here we investigated the role of microbiota and IL-22 in T. gondii-induced small intestinal inflammation. Oral T. gondii infection in B6 mice causes inflammation with IFN. and IL-22 production. In IL-22-deficient mice, T. gondii infection augments the Th1 driven inflammation. Deficiency in either IL-22bp, the soluble IL-22 receptor or Reg3 gamma, an IL-22-dependent antimicrobial lectin/peptide, did not reduce inflammation. Under germ-free conditions, T. gondii-induced inflammation was reduced in correlation with parasite load. But intestinal inflammation is still present in germ-free mice, at low level, in the lamina propria, independently of IL-22 expression. Exacerbated intestinal inflammation driven by absence of IL-22 appears to be independent of IL-22 deficiency associated-dysbiosis as similar inflammation was observed after fecal transplantation of IL-22(-/-) or WT microbiota to germ-free-WT mice. Our results suggest cooperation between parasite and intestinal microbiota in small intestine inflammation development and endogenous IL-22 seems to exert a protective role independently of its effect on the microbiota. In conclusion, IL-22 participates in T. gondii induced acute small intestinal inflammation independently of microbiota and Reg3 gamma.
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https://hal-amu.archives-ouvertes.fr/hal-02143610
Contributor : Ghislain Bidaut <>
Submitted on : Wednesday, May 29, 2019 - 2:50:14 PM
Last modification on : Tuesday, May 26, 2020 - 3:41:01 AM

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A. Couturier-Maillard, N. Froux, J. Piotet-Morin, C. Michaudel, L. Brault, et al.. Interleukin-22-deficiency and microbiota contribute to the exacerbation of Toxoplasma gondii-induced intestinal inflammation. Mucosal Immunology, Nature Pub. Group, 2018, 11 (4), pp.1181-1190. ⟨10.1038/s41385-018-0005-8⟩. ⟨hal-02143610⟩

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