Skip to Main content Skip to Navigation
Journal articles

Phagocyte NADPH oxidase, oxidative stress and lipids: Anti- or pro ageing?

Abstract : The role of NADPH oxidase in ageing is debated because of the dual roles of free radicals, toxic though necessary. In this paper we summarize some results about two aspects linked to the regulation of the activity of phagocyte NADPH oxidase (Nox2), encountered frequently in elderly people: inflammation and hypercholesterolemia. In the presence of a high amount of reactive oxygen species (ROS) created by itself or by any other source, the enzyme activity is mostly lowered. Oxidation of the membrane and/or of one of the cytosolic partners could be responsible for this loss of activity. However using a cell free system, we had also shown that a low amount of ROS could activate this enzyme. Similarly, cholesterol has a similar dual role, either activating or inhibiting. In in vitro cell free system with neutrophil membranes from healthy donors, the addition, as well as the removal of cholesterol, diminishes the Nox2 activity. The activity of Nox2 is lowered in neutrophils of untreated hypercholesterolemic patients. Finally oxysterols (25-hydroxy-cholesterol or 5 alpha, 6 alpha - epoxy-cholesterol) do not induce effects different from that of non-oxidized cholesterol. These findings are in agreement with the Janus role of NADPH oxidase, the main source of non-mitochondrial ROS.
Document type :
Journal articles
Complete list of metadatas

https://hal-amu.archives-ouvertes.fr/hal-02143612
Contributor : Ghislain Bidaut <>
Submitted on : Wednesday, May 29, 2019 - 2:50:20 PM
Last modification on : Thursday, May 30, 2019 - 1:38:47 AM

Identifiers

Collections

Citation

Laura Baciou, Rawand Masoud, Hager Souabni, Xavier Serfaty, Gilda Karimi, et al.. Phagocyte NADPH oxidase, oxidative stress and lipids: Anti- or pro ageing?. MECHANISMS OF AGEING AND DEVELOPMENT, 2018, 172 (SI), pp.30-34. ⟨10.1016/j.mad.2017.11.001⟩. ⟨hal-02143612⟩

Share

Metrics

Record views

41