Skip to Main content Skip to Navigation
Journal articles

Guarani Virophage, a New Sputnik-Like Isolate From a Brazilian Lake

Abstract : Virophages are critical regulators of viral population dynamics and potential actors in the stability of the microbial networks. These small biological entities predate the replicative cycle of giant viruses, such as the members of the Mimiviridae family or their distant relatives, which produce within the cytoplasm of their host cells a viral factory harboring a complex biochemistry propitious to the growth of the smaller parasites. In this paper, we describe the isolation and the characterization of a new virophage, the eighth, that we named Guarani. We observed that Guarani exhibits a late replication cycle compared to its giant virus host. In addition, like all Sputnik strains, Guarani is able to infect the three lineages A, B and C of the Mimiviridae family, and affects the replication and the infectivity of its host virus. In terms of genetic content, Guarani has a 18,967 bp long double-stranded DNA genome encoding 22 predicted genes very similar to Sputnik genes, except for ORF19 and ORF12. The former is more related to Zamilon while the latter seems to be novel. The architecture of the Guarani genome is closely related to Sputnik and Zamilon strains, suggesting a common origin for all these virophages.
Complete list of metadatas

Cited literature [54 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-02159140
Contributor : Isabelle Combe <>
Submitted on : Tuesday, June 18, 2019 - 3:21:13 PM
Last modification on : Thursday, February 6, 2020 - 9:06:08 AM

File

fmicb-10-01003(1).pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Said Mougari, Meriem Bekliz, Jonatas Abrahao, Fabrizio Pinto, Anthony Levasseur, et al.. Guarani Virophage, a New Sputnik-Like Isolate From a Brazilian Lake. Frontiers in Microbiology, Frontiers Media, 2019, 10, ⟨10.3389/fmicb.2019.01003⟩. ⟨hal-02159140⟩

Share

Metrics

Record views

93

Files downloads

171