Characterization of perinatally born glutamatergic neurons of the mouse olfactory bulb based on NeuroD6 expression reveals their resistance to sensory deprivation
Résumé
During postnatal olfactory bulb (OB) neurogenesis, predetermined stem cells residing in the ventricular-subventricular zone continuously generate progenitors that migrate in the rostral migratory stream and integrate into the OB. Although the vast majority of these postnatally generated interneurons are inhibitory, a sub-fraction represents glutamatergic neurons that integrate into the superficial glomerular layer. In the present work, we demonstrate that the bHLH transcription factor NeuroD6 is specifically and transitorily expressed in the dorsal neurogenic lineage that generates glutamatergic juxtaglomerular cells (JGCs) for the OB. Using lineage tracing combined with whole brain clearing, we provide new insight into timing of generation, morphology , and connectivity of glutamatergic JGCs. Specifically, we show that all glutamatergic JGCs send complex axons with varying projection patterns into different layers of the OB. Moreover, we find that, contrary to GABAergic OB interneurons, glutamatergic JGCs survive under sensory deprivation, indicating that inhibitory and excitatory populations are differentially susceptible to environmental stimulation.
Mots clés
Olfactory bulb
NeuroD6 bHLH transcription factor
Postnatal neurogenesis
Glutamatergic neurons
RRID:AB_10013440
RRID:AB_1587367
Sensory deprivation
RRID:AB_2068336
RRID:AB_2200219
RRID:AB_2187539
RRID:AB_2336063
RRID:AB_2749806
RRID:AB_393778
RRID:AB_778267
RRID:AB_828390
RRID:AB_887877
RRID:IMSR_JAX:007914
RRID:MGI:4429523
RRID:MGI:5308867
RRID:MGI:5510844
RRID:SCR_001905
RRID:SCR_002285
RRID:SCR_007370
RRID:SCR_013672
RRID:AB_2619904
Loading...