HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis

Abstract : Despite recent advances in understanding the biogenesis of iron-sulfur (Fe-S) proteins, most studies focused on aerobic bacteria as model organisms. Accordingly, multiple players have been proposed to participate in the Fe-S delivery step to apo-target proteins, but critical gaps exist in the knowledge of Fe-S proteins biogenesis in anaerobic organisms. Mrp/NBP35 ATP-binding proteins are a subclass of the soluble P-loop containing nucleoside triphosphate hydrolase superfamily (P-loop NTPase) known to bind and transfer Fe-S clusters in vitro. Here, we report investigations of a novel atypical two-domain Mrp/NBP35 ATP-binding protein named Mrp ORP associating a P-loop NTPase domain with a dinitrogenase iron-molybdenum cofactor biosynthesis domain (Di-Nase). Characterization of full length Mrp ORP , as well as of its two domains, showed that both domains bind Fe-S clusters. We provide in vitro evidence that the P-loop NTPase domain of the Mrp ORP can efficiently transfer its Fe-S cluster to apo-target proteins of the ORange Protein (ORP) complex, suggesting that this novel protein is involved in the maturation of these Fe-S proteins. Last, we showed for the first time, by fluorescence microscopy imaging a polar localization of a Mrp/NBP35 protein.
Document type :
Journal articles
Complete list of metadata

Cited literature [55 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-02186929
Contributor : Delphine Leroi Connect in order to contact the contributor
Submitted on : Wednesday, July 17, 2019 - 4:05:11 PM
Last modification on : Tuesday, March 29, 2022 - 3:44:01 PM

File

pardoux_scirep.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Romain Pardoux, Anouchka Fiévet, Cíntia Carreira, Céline Brochier-Armanet, Odile Valette, et al.. The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis. Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.360-373. ⟨10.1038/s41598-018-37021-8⟩. ⟨hal-02186929⟩

Share

Metrics

Record views

56

Files downloads

73