Skip to Main content Skip to Navigation
New interface
Journal articles

A small RNA controls bacterial sensitivity to gentamicin during iron starvation

Abstract : Phenotypic resistance describes a bacterial population that becomes transiently resistant to an antibiotic without requiring a genetic change. We here investigated the role of the small regulatory RNA (sRNA) RyhB, a key contributor to iron homeostasis, in the phenotypic resistance of Escherichia coli to various classes of antibiotics. We found that RyhB induces phe-notypic resistance to gentamicin, an aminoglycoside that targets the ribosome, when iron is scarce. RyhB induced resistance is due to the inhibition of respiratory complexes Nuo and Sdh activities. These complexes, which contain numerous Fe-S clusters, are crucial for generating a proton motive force (pmf) that allows gentamicin uptake. RyhB regulates negatively the expression of nuo and sdh, presumably by binding to their mRNAs and, as a consequence, inhibiting their translation. We further show that Isc Fe-S biogenesis machinery is essential for the maturation of Nuo. As RyhB also limits levels of the Isc machinery, we propose that RyhB may also indirectly impact the maturation of Nuo and Sdh. Notably, our study shows that respiratory complexes activity levels are predictive of the bacterial sensitivity to gentamicin. Altogether, these results unveil a new role for RyhB in the adaptation to antibiotic stress, an unprecedented consequence of its role in iron starvation stress response.
Document type :
Journal articles
Complete list of metadata

Cited literature [55 references]  Display  Hide  Download
Contributor : Delphine LEROI Connect in order to contact the contributor
Submitted on : Thursday, July 18, 2019 - 12:11:01 PM
Last modification on : Wednesday, June 1, 2022 - 9:36:08 AM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution 4.0 International License




Sylvia Chareyre, Frédéric Barras, Pierre Mandin. A small RNA controls bacterial sensitivity to gentamicin during iron starvation. PLoS Genetics, 2019, 15 (4), pp.e1008078. ⟨10.1371/journal.pgen.1008078⟩. ⟨hal-02188209⟩



Record views


Files downloads