Skip to Main content Skip to Navigation
Journal articles

Juxtaposed membranes underpin cellular adhesion and display unilateral cell division of multicellular magnetotactic prokaryotes

Abstract : Multicellular magnetotactic prokaryotes (MMPs) exhibit peculiar coordination of swimming along geomagnetic field lines. Approximately 40–80 cells assemble, with a helical geometry or axisymmetry, into spherical or ellipsoidal MMPs respectively. To contribute to a comprehensive understanding of bacterial multicellularity here we took multiple microscopic approaches to study the diversity, assembly, reproduction and motility of ellipsoidal MMPs. Using correlative fluorescence in situ hybridization and scanning electron microscopy analysis, we found an unexpected diversity in populations of ellipsoidal MMPs in the Mediterranean Sea. The high‐pressure freezing/freeze substitution fixation technique allowed us to show, for the first time, that cells adhere via juxtaposed membranes and are held together by a rimming lattice. Fluorescence confocal microscopy and ultrathin section images revealed not only the one‐layer hollow three‐dimensional architecture, but also periphery–core unilateral constriction of constituent cells and unidirectional binary fission of the ellipsoidal MMPs. This finding suggests the evolution toward MMPs multicellularity via the mechanism of incomplete separation of offspring. Remarkably, thousands of flagellar at the periphery surface of cells underpin the coordinated swimming of MMPs in response to mechanical, chemical, magnetic and optical stimuli, including a magnetotactic photokinesis behaviour. Together these results unveil the unique structure and function property of ellipsoidal MMPs.
Document type :
Journal articles
Complete list of metadatas

Cited literature [115 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-02200833
Contributor : Delphine Leroi <>
Submitted on : Monday, February 10, 2020 - 12:19:52 PM
Last modification on : Tuesday, September 1, 2020 - 3:44:16 PM
Long-term archiving on: : Monday, May 11, 2020 - 2:54:24 PM

File

qiansantini.pdf
Files produced by the author(s)

Identifiers

Citation

Xin‐xin Qian, Claire‐lise Santini, Artemis Kosta, Nicolas Menguy, Hugo Le Guenno, et al.. Juxtaposed membranes underpin cellular adhesion and display unilateral cell division of multicellular magnetotactic prokaryotes. Environmental Microbiology, Wiley-Blackwell, 2020, 141 (13), pp.5555-5562. ⟨10.1111/1462-2920.14710⟩. ⟨hal-02200833⟩

Share

Metrics

Record views

154

Files downloads

235