Skip to Main content Skip to Navigation
Journal articles

Draft genome and description of Cohnella massiliensis sp. nov., a new bacterial species isolated from the blood culture of a hemodialysis patient

Abstract : Strain 6021052837T was isolated from the blood culture of a hemodialysis patient on Chocolat PolyViteX medium at 37 °C after 2 days of incubation. Colonies could not be identified by our systematic MALDI-TOF Mass Spectrometry screening. The16S rRNA gene sequencing showed that the strain had 96% sequence identity with Cohnella formosensis (Genbank accession number JN806384), the phylogenetic closely related type strain of a species with standing in nomenclature, which putatively classifies it as a new species. The colonies cultivated on Columbia agar with 5% sheep blood medium at 37 °C after 24 h of incubation, are white pigmented, their size varied from 1.5 to 2 mm in diameter. Strain 6021052837T is an aerobic, Gram-negative, motile, spore forming rod, which cannot grow microaerophilically or under anaerobic conditions. The major fatty acids are branched saturated fatty acids: 14-methyl-pentadecanoic acid (34%) and 12-methyl-tetradecanoic acid (31%). The 6.328 Mb long genome, composed of 25 contigs, has a G+C content of 57.24%. Out of the 5710 predicted genes, 5646 were protein-coding genes and 64 were RNAs. A total of 3239 genes (57.37%) were assigned as putative function (by COGs) and 288 genes were identified as ORFans (5.1%). Average genomic identity of orthologous gene sequences (AGIOS) of strain 6021052837T against genomes of the type strains of related species ranged between 58.26 and 79.63%, respectively. According to our taxonogenomics results, we propose the creation of Cohnella massiliensis sp. nov. that contains the type strain 6021052837T (= CSUR P2659, =DSM103435).
Document type :
Journal articles
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-02202136
Contributor : Isabelle Combe <>
Submitted on : Friday, September 17, 2021 - 8:30:03 PM
Last modification on : Tuesday, September 21, 2021 - 3:35:16 AM

File

RAA2018.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Rita Abou Abdallah, Jacques Bou Khalil, Claudia Andrieu, Enora Tomei, Nicholas Armstrong, et al.. Draft genome and description of Cohnella massiliensis sp. nov., a new bacterial species isolated from the blood culture of a hemodialysis patient. Archives of Microbiology, Springer Verlag, 2019, 201 (3), pp.305-312. ⟨10.1007/s00203-018-1612-1⟩. ⟨hal-02202136⟩

Share

Metrics

Record views

97

Files downloads

18