Pru p 7 sensitization is a predominant cause of severe, cypress pollen‐associated peach allergy

Caroline Klingebiel (1) , Yannick Chantran (2, 3) , Rihane Arif‐lusson (4, 5) , Angelica Ehrenberg (6) , Jonas Östling (6) , Alain Poisson (7) , Valérie Liabeuf (8) , Chantal Agabriel (9) , Joëlle Birnbaum (10) , Françoise Porri (7) , Anne Sarrat (11) , Pol-André Apoil (12) , Mylène Vivinus (13) , Lorna Garnier (14) , Anca Mirela Chiriac (15, 16, 17) , Davide‐paolo Caimmi (15, 17, 18) , Jean‐luc Bourrain (15, 18) , Pascal Demoly (18, 17, 15) , Stéphane Guez (19) , Franck Boralevi (20) , Bertrand Lovato (21) , Céline Palussière (22) , Sylvie Leroy (23) , Thierry Bourrier (24) , Lisa Giovannini‐chami (24) , Marion Gouitaa (25) , Agnès Aferiat‐derome (26) , Denis Charpin (27) , Tünde Sofalvi (25) , Isabelle Cabon‐boudard (25) , Yann‐patrick Massabie‐bouchat (26) , Bernard Hofmann (28) , Nathalie Bonardel (15) , Mireille Dron‐gonzalvez (29) , Benoît Sterling (27) , Ania Carsin (27) , Serge Vivinus (23) , Bernard Poitevin (30) , Laureline Nicolau (31) , Geneviève Liautard (32) , Christophe Soler (1) , Soraya Mezouar (4) , Isabella Annesi-Maesano (33) , Jean-Louis Mege (5, 4) , Jonas Lidholm (6) , Joana Vitte (5, 4)

1 Laboratoire Synlab Provence
2 Laboratoire d'Hématologie et d'Immunologie [CHU Saint-Antoine]
3 UMRS893 - Centre de Recherche Saint-Antoine
4 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
5 MEPHI - Microbes évolution phylogénie et infections
6 Thermo Fisher Scientific (Bremen) GmbH
7 Hôpital Saint-Joseph [Marseille]
8 Service de dermatologie, vénéreologie et cancérologie cutanée [Hôpital de la Timone - APHM]
9 Service de pédiatrie multidisciplinaire [Hôpital de la Timone Enfants - APHM]
10 CHIAP - Centre Hospitalier d'Aix en Provence [Aix-en-Provence]
11 Service d'immunologie et d'immunogénétique [Bordeaux]
12 Institut Fédératif de Biologie (IFB)
13 CHU - Hôpital Archet 2 [Nice]
14 Service d'immunologie [Centre Hospitalier Lyon Sud - HCL]
15 Département pneumologie et addictologie [Montpellier]
16 UM - Université de Montpellier
17 iPLESP - Institut Pierre Louis d'Epidémiologie et de Santé Publique
18 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
19 CHU Bordeaux [Bordeaux]
20 Hôpital Pellegrin
21 Centre Médical de Mérignac
22 Centre Médical de Cenon
23 CHU - Hôpital Pasteur [Nice]
24 CHU-Lenval - Hôpitaux Pédiatriques de Nice Lenval
25 APHM - Assistance Publique - Hôpitaux de Marseille
26 Espace Médical Castellane
27 TIMONE - Hôpital de la Timone [CHU - APHM]
28 Centre Hospitalier de Carpentras
29 Centre Hospitalier de Martigues
30 Centre hospitalier de Bormes-les-Mimosas
31 Centre Hospitalier Saint Jean de Perpignan
32 Centre Hospitalier de Solliès-Pont
33 Epidémiologie, Systèmes d'Information, Modélisation

BACKGROUND: Peach is a common elicitor of food allergic reactions. Peach-induced immediate reactions may occur as benign pollen-food syndromes, usually due to birch pollen-related PR-10 cross-reactivity in temperate climates, and as potentially severe primary food allergies, predominantly related to nsLTP Pru p 3 in Mediterranean regions. The newly described peach allergen Pru p 7 has gained recent attention as a potential peach allergy severity marker. Sensitization to Pru p 7 and its allergenic homologues of the gibberellin-regulated protein family occurs in areas with high Cupressaceae tree pollen exposure. OBJECTIVE: We sought to investigate the distribution, clinical characteristics and molecular associations of Pru p 7 sensitization among subjects with suspected peach allergy in different regions of France. METHODS: Subjects with suspected peach allergy (n = 316) were included. Diagnostic work-up was performed according to current guidelines, including open food challenge when required. IgE antibody measurements and competition experiments were performed using the ImmunoCAP assay platform. RESULTS: Sensitization to Pru p 7 was present in 171 (54%) of all subjects in the study and in 123 of 198 (62%) diagnosed as peach allergic, more than half of whom were sensitized to no other peach allergen. Frequency and magnitude of Pru p 7 sensitization were associated with the presence of peach allergy, the clinical severity of peach-induced allergic reactions and the level of cypress pollen exposure. Cypress pollen extract completely outcompeted IgE binding to Pru p 7. Pru p 7 was extremely potent in basophil activation tests. CONCLUSION AND CLINICAL RELEVANCE: A subtype of Cupressaceae pollinosis, characterized by Pru p 7 sensitization, can be an underlying cause of severe peach allergy.

Keywords: peamaclein; Pru p 7; allergens and epitopes; anaphylaxis; basophil; cypress pollinosis; food allergy; IgE; immunological tests

Domains: Life Sciences [q-bio]; Immunology

Caroline Klingebiel, Yannick Chantran, Rihane Arif‐lusson, Angelica Ehrenberg, Jonas Östling, et al.. Pru p 7 sensitization is a predominant cause of severe, cypress pollen‐associated peach allergy. Clinical and Experimental Allergy, 2019, 49 (4), pp.526-536. ⟨10.1111/cea.13345⟩. ⟨hal-02202464⟩