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Role and recruitment of the TagL peptidoglycan-binding protein during Type VI secretion system biogenesis

Abstract : The type VI secretion system (T6SS) is an injection apparatus that uses a springlike mechanism for effector delivery. The contractile tail is composed of a needle tipped by a sharpened spike and wrapped by the sheath that polymerizes in an extended conformation on the assembly platform, or baseplate. Contraction of the sheath propels the needle and effectors associated with it into target cells. The passage of the needle through the cell envelope of the attacker is ensured by a dedicated trans-envelope channel complex. This membrane complex (MC) comprises the TssJ lipoprotein and the TssL and TssM inner membrane proteins. MC assembly is a hierarchized mechanism in which the different subunits are recruited in a specific order: TssJ, TssM, and then TssL. Once assembled, the MC serves as a docking station for the baseplate. In enteroaggregative Escherichia coli, the MC is accessorized by TagL, a peptidoglycan-binding (PGB) inner membrane-anchored protein. Here, we show that the PGB domain is the only functional domain of TagL and that the N-terminal transmembrane region mediates contact with the TssL transmembrane helix. Finally, we conduct fluorescence microscopy experiments to position TagL in the T6SS biogenesis pathway, demonstrating that TagL is recruited to the membrane complex downstream of TssL and is not required for baseplate docking.
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Submitted on : Thursday, October 31, 2019 - 10:51:31 AM
Last modification on : Tuesday, November 5, 2019 - 1:34:00 AM
Long-term archiving on: : Saturday, February 1, 2020 - 2:55:30 PM

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Yoann Santin, Claire Camy, Abdelrahim Zoued, Thierry Doan, Marie-Stephanie Aschtgen, et al.. Role and recruitment of the TagL peptidoglycan-binding protein during Type VI secretion system biogenesis. Journal of Bacteriology, American Society for Microbiology, 2019, ⟨10.1128/JB.00173-19⟩. ⟨hal-02341043⟩

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