Real-817 space refinement in PHENIX for cryo-EM and crystallography, Acta Crystallogr. Sect. D Struct, 2018. ,
, Biol, vol.74, pp.531-544
, Pyem. GitHub Available, 2016.
, SciN Is an Outer, vol.821, 2008.
, Membrane Lipoprotein Required for Type VI Secretion in Enteroaggregative Escherichia coli
, Bacteriol, vol.190, pp.7523-7531
, SciN Is an Outer, vol.824, 2008.
, Membrane Lipoprotein Required for Type VI Secretion in Enteroaggregative Escherichia coli
, Bacteriol, vol.190, pp.7523-7531
The C-tail anchored TssL subunit, an 827 essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, 2012. ,
Type VI secretion requires a 830 dynamic contractile phage tail-like structure, Nature, vol.483, pp.182-186, 2012. ,
Dissecting the bacterial type VI 832 secretion system by a genome wide in silico analysis: what can be learned from available 833 microbial genomic resources?, BMC Genomics, vol.10, p.104, 2009. ,
, An Epigenetic Switch Involving, vol.835, 2011.
, Overlapping Fur and DNA Methylation Optimizes Expression of a Type VI Secretion Gene 836 Cluster, PLoS Genet, vol.7, p.1002205
Imaging Type VI Secretion-838, 2013. ,
, Mediated Bacterial Killing. Cell Rep, vol.3, pp.36-41
Type VI secretion and bacteriophage tail tubes share a 840 common assembly pathway, EMBO Rep, vol.15, pp.315-321, 2014. ,
, The Type VI Secretion TssEFGK-VgrG, 2015.
URL : https://hal.archives-ouvertes.fr/hal-01778563
, Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves 843
, As Assembly Platform for Tail Tube/Sheath Polymerization, PLOS Genet, vol.11, p.1005545
, , p.845, 2013.
Using Evolutionary Data to Raise Testable Hypotheses about Protein Function, Isr. J, vol.846 ,
, Chem, vol.53, pp.199-206
Crystal structure of the bacterial type VI secretion system component system revealed by electron cryotomography, EMBO Rep, vol.18, pp.1090-1099, 2015. ,
A rapid method for efficient gene replacement in the 852 filamentous fungus Aspergillus nidulans, Nucleic Acids Res, vol.28, p.97, 2000. ,
, , vol.854
MolProbity : all-atom structure validation for macromolecular 855 crystallography, Acta Crystallogr. Sect. D Biol. Crystallogr, vol.66, pp.12-21, 2010. ,
, , p.857
Biogenesis and structure of a type VI secretion 858 baseplate, Nat. Microbiol, vol.3, pp.1404-1416, 2018. ,
, Atomic Structure of T6SS Reveals Interlaced, vol.860, 2015.
, Array Essential to Function. Cell, vol.160, pp.940-951
Cryo-EM single particle analysis with the Volta phase plate, Elife, vol.5, p.862, 2016. ,
One-step inactivation of chromosomal genes in Escherichia coli K-863 12 using PCR products, Proc. Natl. Acad. Sci, vol.97, pp.6640-6645, 2000. ,
MicrobeJ, a tool for high throughput bacterial cell 865 detection and quantitative analysis, Nat. Microbiol, vol.1, p.16077, 2016. ,
, , vol.867
Biogenesis 868 and structure of a type VI secretion membrane core complex, Nature, vol.523, pp.555-560, 2015. ,
, , p.870, 2012.
, Structural characterization and oligomerization of the TssL protein, a component shared by 871 bacterial type VI and type IVb secretion systems, J. Biol. Chem, vol.287, pp.14157-68
Features and development of Coot, Crystallogr. Sect. D Biol. Crystallogr, vol.873, pp.486-501, 2010. ,
, , vol.875
Towards a structural comprehension of bacterial type VI secretion systems: 876 characterization of the TssJ-TssM complex of an Escherichia coli pathovar, PLoS Pathog, vol.7, p.877, 2011. ,
, , vol.879
A phospholipase A 1 antibacterial Type VI secretion 880 effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery, 2016. ,
, Mol. Microbiol, vol.99, pp.1099-1118
Controlled bacterial lysis for electron 883 tomography of native cell membranes, Structure, vol.22, pp.1875-1882, 2014. ,
Van den Broeck I, Troupiotis-Tsaïlaki A, Jonckheere 889 CTS1 Type VI Secretion System in Interbacterial Competition, Appl. Environ. Microbiol, vol.79, p.38 ,
Type VI secretion and anti-host effectors, Curr. Opin, 2016. ,
, , vol.29, pp.81-93
A technique for improved focused ion beam 894 milling of cryo-prepared life science specimens, J. Microsc, vol.226, pp.263-272, 2007. ,
, Situ Molecular Architecture of the Salmonella 896, 2017.
, Type III Secretion Machine. Cell, vol.168, pp.1065-1074
Automated builder and database of protein/membrane complexes for 898 molecular dynamics simulations, PLoS One, vol.2, 2007. ,
Template-based protein structure 900 modeling using the RaptorX web server, Nat. Protoc, vol.7, pp.1511-1522, 2012. ,
The Phyre2 web portal for protein 902 modeling, prediction and analysis, Nat. Protoc, vol.10, pp.845-858, 2015. ,
Computer Visualization of Three-Dimensional 904, 1996. ,
, Image Data Using IMOD, J. Struct. Biol, vol.116, pp.71-76
Computer Visualization of Three-Dimensional 906, 1996. ,
, Image Data Using IMOD, J. Struct. Biol, vol.116, pp.71-76
Inference of Macromolecular Assemblies from Crystalline State, J. Mol, 2007. ,
, Biol, vol.372, pp.774-797
,
, Structure of the Type VI Secretion System Contractile Sheath. 911 Cell, vol.160, pp.952-962, 2015.
Methods for generating precise deletions and insertions in the 913 genome of wild-type Escherichia coli: application to open reading frame characterization, 1997. ,
, Bacteriol, vol.179, pp.6228-6237
Molecular Dissection of the 916 Interface between the Type VI Secretion TssM Cytoplasmic Domain and the TssG Baseplate 917 Component, J. Mol. Biol, vol.428, pp.4424-4437, 2016. ,
An IcmF Family Protein, ImpLM, Is an Integral Inner Membrane 919, 2009. ,
, Protein Interacting with ImpKL, and Its Walker A Motif Is Required for Type VI Secretion System-920
, Mediated Hcp Secretion in Agrobacterium tumefaciens, J. Bacteriol, vol.191, pp.4316-4329
Focused-ion-beam thinning of frozen-922 hydrated biological specimens for cryo-electron microscopy, Nat. Methods, vol.4, pp.215-217, 2007. ,
, , 2016.
Automated electron microscope tomography using robust prediction of 925 specimen movements, J. Struct. Biol, vol.152, pp.36-51, 2005. ,
Imaging bacteria inside their host by cryo-focused ion 927 beam milling and electron cryotomography, Curr. Opin. Microbiol, vol.43, pp.62-68, 2018. ,
Robust workflow and 929 instrumentation for cryo-focused ion beam milling of samples for electron cryotomography, 930 Ultramicroscopy, vol.190, pp.1-11, 2018. ,
A virulence locus of Pseudomonas 933 aeruginosa encodes a protein secretion apparatus, Science, vol.312, pp.1526-1556, 2006. ,
, , p.935
, AA (2011) REFMAC 5 for the refinement of macromolecular crystal structures, Acta Crystallogr
, Sect. D Biol. Crystallogr, vol.67, pp.355-367
Cryo-EM 938 reconstruction of Type VI secretion system baseplate and sheath distal end, EMBO J, vol.37, p.97103, 2018. ,
The Molecular Architecture of Axonemes Revealed by Cryoelectron Tomography, 2006. ,
,
Protein structure determination using metagenome sequence data. Science (80-. 944 ), vol.355, pp.294-298, 2017. ,
, Meng EC & Ferrin TE, 2004.
, Chimera--a visualization system for exploratory research and analysis, J. Comput. Chem, vol.25, pp.947-1605
cryoSPARC: algorithms for rapid 949 unsupervised cryo-EM structure determination, Nat. Methods, vol.14, pp.290-296, 2017. ,
Mechanism of 951 loading and translocation of type VI secretion system effector Tse6, Nat. Microbiol, vol.3, p.1152, 2018. ,
Automatic local resolution-based 954 sharpening of cryo-EM maps, pp.1-21, 2018. ,
, Structure of the T6SS lipoprotein TssJ1 from 962, 2013.
, Pseudomonas aeruginosa, Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun, vol.69, pp.607-617
The structure of the conserved type six secretion protein 964, 2012. ,
, TssL (DotU) from Francisella novicida, J. Mol. Biol
Optimal Determination of Particle Orientation, Absolute Hand, 966 and Contrast Loss in Single-particle Electron Cryomicroscopy, J. Mol. Biol, vol.333, pp.721-745, 2003. ,
Type VI secretion delivers 968 bacteriolytic effectors to target cells, Nature, vol.475, pp.343-347, 2011. ,
Type VI secretion system effectors: Poisons with a 970 purpose, Nat. Rev. Microbiol, vol.12, pp.137-148, 2014. ,
RELION: implementation of a Bayesian approach to cryo-EM structure 972 determination, J. Struct. Biol, vol.180, pp.519-549, 2012. ,
NIH Image to ImageJ: 25 years of image analysis, Nat. 974 Methods, vol.9, pp.671-676, 2012. ,
PAAR-repeat proteins 976 sharpen and diversify the type VI secretion system spike, Nature, vol.500, pp.350-353, 2013. ,
, , p.978, 2013.
, Haemolysin Coregulated Protein Is an Exported Receptor and Chaperone of Type VI Secretion 979
, Mol. Cell
HOLE: A program for the analysis 981 of the pore dimensions of ion channel structural models, J. Mol. Graph, vol.14, pp.354-360, 1996. ,
, , p.983
Identification of the gate regions in the primary structure of the 984 secretin pIV, Mol. Microbiol, vol.76, pp.133-150, 2010. ,
Automated map sharpening by 986 maximization of detail and connectivity, Acta Crystallogr. Sect. D Struct. Biol, vol.74, pp.545-559, 2018. ,
Adaptor Proteins of Type VI Secretion System Effectors, 2017. ,
, Trends Microbiol, vol.25, pp.8-10
Cryo-EM structure of the extended type VI secretion system sheath-tube complex, 2017. ,
, , vol.2, pp.1507-1512
Automated structure refinement 993 of macromolecular assemblies from cryo-EM maps using Rosetta, Elife, vol.5, 2016. ,
Crystal structure of the periplasmic 995 domain of TssL, a key membrane component of Type VI secretion system, Int. J. Biol. Macromol, vol.996, issue.120, pp.1474-1479, 2018. ,
In situ imaging of bacterial secretion systems by electron 998 cryotomography, Methods in Molecular Biology pp, pp.353-375, 2017. ,
Structural insights into the secretin translocation channel in 1000 the type II secretion system, Nat. Struct. Mol. Biol, vol.24, pp.177-183, 2017. ,
Surette MG & Alon U 1002 (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli, Nat. 1003 Methods, vol.3, pp.623-628 ,
Gctf: Real-time CTF determination and correction, J. Struct. Biol, vol.193, pp.1-12, 2016. ,
Antagonism toward the intestinal microbiota and 1006 its effect on Vibrio cholerae virulence. Science (80-. ), vol.359, pp.210-213, 2018. ,
UCSF 1008 tomography: An integrated software suite for real-time electron microscopic tomographic data 1009 collection, alignment, and reconstruction, J. Struct. Biol, vol.157, pp.138-147, 2007. ,
MotionCor2: anisotropic 1011 correction of beam-induced motion for improved cryo-electron microscopy, Nat. Methods, vol.14, pp.1012-331, 2017. ,
, , p.1014, 2016.
, Structure-Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction 1015 between the Type VI Secretion Baseplate and Membrane Complexes, J. Mol. Biol, vol.428, p.4423
Tryptophan-1018 mediated Dimerization of the TssL Transmembrane Anchor Is Required for Type VI Secretion 1019 System Activity, J. Mol. Biol, 2018. ,
TssK 1021 is a trimeric cytoplasmic protein interacting with components of both phage-like and 1022 membrane anchoring complexes of the type VI secretion system, J. Biol. Chem, vol.288, p.27041, 2013. ,