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Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus

Abstract : It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
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Submitted on : Tuesday, November 19, 2019 - 11:39:22 AM
Last modification on : Saturday, October 3, 2020 - 3:26:37 AM
Long-term archiving on: : Thursday, February 20, 2020 - 5:38:35 PM

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K. Poncin, A. Roba, R. Jimmidi, G. Potemberg, A. Fioravanti, et al.. Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus. Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-12516-8⟩. ⟨hal-02368673⟩

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