Translocation of Mycobacterium tuberculosis after experimental ingestion

Mustapha Fellag (1) , Ahmed Loukil (1) , Jamal Saad (1) , Hubert Lepidi (1) , Feriel Bouzid (1) , Fabienne Bregeon (1) , Michel Drancourt (1)

1 MEPHI - Microbes évolution phylogénie et infections

Human tuberculosis is a life-threatening infection following the inhalation of Mycobacterium tuberculosis, while the closely related bacteria Mycobacterium bovis and Mycobacterium canettii are thought to be transmitted by ingestion. To explore whether M. tuberculosis could also infect individuals by ingestion, male BALBc mice were fed 2 x 10 6 CFUs of M. tuberculosis Beijing or phosphate-buffered saline as a negative control, over a 28-day experiment. While eight negative control mice remained disease-free, M. tuberculosis was identified in the lymph nodes and lungs of 8/14 mice and in the spleens of 4/14 mice by microscopy, PCR-based detection and culture. Whole-genome sequencing confirmed the identity of the inoculum and the tissue isolates. In these genetically identical mice, the dissemination of M. tuberculosis correlated with the results of the culture detection of four intestinal bacteria. These observations indicate that ingested M. tuberculosis mycobacteria can translocate, notably provoking lymphatic tuberculosis.

Mots clés: Mycobacterium tuberculosis; Lymph nodes; Mouse models; Gastrointestinal tract; Lungs; Spleen; Fluorescent in situ hybridization; DNA extraction

Domaines: Bactériologie; Virologie; Parasitologie; Cardiologie et système cardiovasculaire; Maladies émergentes; Maladies infectieuses

Mustapha Fellag, Ahmed Loukil, Jamal Saad, Hubert Lepidi, Feriel Bouzid, et al.. Translocation of Mycobacterium tuberculosis after experimental ingestion. PLoS ONE, 2019, 14 (12), pp.e0227005. ⟨10.1371/journal.pone.0227005⟩. ⟨hal-02446276⟩