ECM - École Centrale de Marseille (Pôle de l'étoile - Technopole de Château-Gombert - 38 rue Frédéric Joliot-Curie - 13013 Marseille - France)
AMU - Aix Marseille Université : UMR7249/ UMR 6133 (Aix-Marseille Université
Jardins du Pharo
58 Boulevard Charles Livon
13284 Marseille cedex 7 - France)
Abstract : Purpose of review: Highly coordinated cellular interactions occur in the healthy or pathologic adult rodent central nervous system (CNS). Until recently, technical challenges have restricted the analysis of these events to largely static modes of study such as immuno-fluorescence and electron microscopy on fixed tissues. The development of intravital imaging with subcellular resolution is required to probe the dynamics of these events in their natural context, the living brain. Recent findings: This review focuses on the recently developed live non-linear optical imaging modalities, the core principles involved, the identified technical challenges that limit their use and the scope of their applications. We highlight some practical applications for these modalities with a specific attention given to Experimental Autoimmune Encephalomyelitis (EAE), a rodent model of a chronic inflammatory disease of the CNS characterized by the formation of disseminated demyelinating lesions accompanied by axonal degeneration. Summary: We conclude that label-free nonlinear optical imaging combined to two photon imaging will continue to contribute richly to comprehend brain function and pathogenesis and to develop effective therapeutic strategies.
https://hal-amu.archives-ouvertes.fr/hal-02450866 Contributor : Genevieve RougonConnect in order to contact the contributor Submitted on : Thursday, January 23, 2020 - 10:30:26 AM Last modification on : Wednesday, November 3, 2021 - 7:28:53 AM Long-term archiving on: : Friday, April 24, 2020 - 1:17:38 PM
Genevieve Rougon, Sophie Brasselet, Franck Debarbieux. Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents. Brain Plasticity, IOS Press, 2016, 2, pp.31 - 48. ⟨10.3233/BPL-160028⟩. ⟨hal-02450866⟩