T. Shi, R. H. Reeves, D. A. Gilichinsky, and E. I. Friedmann, Characterization of Viable Bacteria from, 1997.

, Siberian Permafrost by 16S rDNA Sequencing, Microb Ecol, vol.33, pp.169-179


T. Vishnivetskaya, S. Kathariou, J. Mcgrath, D. Gilichinsky, and J. M. Tiedje, Low-temperature 542 recovery strategies for the isolation of bacteria from ancient permafrost sediments, Extremophiles, vol.543, pp.165-173, 2000.

D. E. Graham, M. D. Wallenstein, T. A. Vishnivetskaya, M. P. Waldrop, T. J. Phelps et al., , p.545

L. G. Whyte, E. M. Rivkina, D. A. Gilichinsky, D. A. Elias, R. Mackelprang et al., , p.546

D. Wagner, S. D. Wullschleger, and J. K. Jansson, Microbes in thawing permafrost: the unknown 547 variable in the climate change equation, ISME J, vol.6, pp.709-712, 2012.

C. Abergel, M. Legendre, and J. M. Claverie, The rapidly expanding universe of giant viruses: Mimivirus, p.561

P. Pandoravirus and M. , FEMS Microbiol Rev, vol.39, pp.779-796, 2015.


A. Levasseur, J. Andreani, J. Delerce, B. Khalil, J. Robert et al., Comparison 564 of a Modern and Fossil Pithovirus Reveals Its Genetic Conservation and Evolution, Genome Biol, vol.565, 2016.

, Evol, vol.8, pp.2333-2339

J. Andreani, S. Aherfi, B. Khalil, J. Y. , D. Pinto et al., , 2016.

. Cedratvirus, Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses, Viruses, vol.568, p.300

C. Bertelli, L. Mueller, V. Thomas, T. Pillonel, N. Jacquier et al., Cedratvirus lausannensis -570 digging into Pithoviridae diversity, Environ Microbiol, vol.19, pp.4022-4034, 2017.


E. R. Quemin, S. Corroyer-dulmont, A. Baskaran, E. Penard, A. D. Gazi et al., , p.573

C. Abergel and J. Krijnse-locker, Complex membrane remodeling during virion assembly of the 574 30,000-year-old Mollivirus sibericum, J Virol, vol.93, issue.13, pp.388-407, 2019.


M. Legendre, J. M. Alempic, P. N. Lartigue, A. Jeudy, S. Poirot et al., , p.585

C. Abergel and J. M. Claverie, Pandoravirus celtis illustrates the microevolution processes at work 586 in the giant Pandoraviridae genomes, Front Microbiol, vol.10, p.430, 2019.

, NCBI Resource Coordinators, Database resources of the National Center for, 2018.

, Biotechnology Information. Nucleic Acids Res, vol.46, pp.8-13

A. J. Heidel, H. M. Lawal, M. Felder, C. Schilde, N. R. Helps et al., , p.591

L. Eichinger, M. Platzer, A. A. Noegel, P. Schaap, and G. Glöckner, Phylogeny-wide analysis of social 592 amoeba genomes highlights ancient origins for complex intercellular communication, Genome Res, vol.593, pp.1882-1891, 2011.

V. Thomas, C. Bertelli, F. Collyn, N. Casson, A. Telenti et al., , 2011.

, Lausannevirus, a giant amoebal virus encoding histone doublets, Environ Microbiol, vol.13, pp.1454-1466


F. Maumus and G. Blanc, Study of gene trafficking between acanthamoeba and giant viruses 598 suggests an undiscovered fof amoeba-infecting viruses, Genome Biol, p.599, 2016.

, Evol, vol.8, pp.3351-3363

N. Chelkha, A. Levasseur, P. Pontarotti, D. Raoult, B. Scola et al., Distant Mimivirus relative with a 610 larger genome highlights the fundamental features of Megaviridae, Proc Natl Acad Sci, vol.108, pp.17486-17491, 2011.

A. Bankevich, S. Nurk, D. Antipov, A. Gurevich, M. Dvorkin et al.,

S. , P. A. Pyshkin, A. Sirotkin, A. Vyahhi, N. Tesler et al., , 2012.

, New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing, J Comput Biol, vol.615, pp.455-477

N. A. Dunn, D. R. Unni, C. Diesh, M. Munoz-torres, N. L. Harris et al., , p.617

S. Lewis, Apollo: Democratizing genome annotation, PLoS Comput Biol, vol.15, p.1006790, 2019.


A. Marchler-bauer, M. K. Derbyshire, N. R. Gonzales, S. Lu, F. Chitsaz et al.,

M. Hurwitz, D. I. Lanczycki, C. J. Lu, F. Marchler, G. H. Song et al., , p.621

C. Zheng and S. H. Bryant, CDD: NCBI's conserved domain database, Nucleic Acids Res, vol.43, pp.222-622, 2015.

M. Remmert, A. Biegert, A. Hauser, and J. Söding, HHblits: lightning-fast iterative 624 protein sequence searching by HMM-HMM alignment, Nat Methods, vol.9, pp.173-175, 2011.

, Trends Genet, vol.16, pp.2024-2026

S. Andrews, FastQC: a quality control tool for high throughput sequence data, Available, vol.637, 2010.

B. Bushnell, J. Rood, and E. Singer, BBMerge -Accurate paired shotgun read merging via 639 overlap, PLoS ONE, vol.12, 2017.

D. Li, C. Liu, R. Luo, K. Sadakane, and T. Lam, MEGAHIT: An ultra-fast single-node solution 641 for large and complex metagenomics assembly via succinct de Bruijn graph, Bioinformatics, vol.642, pp.1674-1676, 2015.

B. Langmead and S. L. Salzberg, Fast gapped-read alignment with Bowtie 2, Nat Methods, vol.644, pp.357-359, 2012.

L. T. Nguyen, H. A. Schmidt, V. Haeseler, A. Minh, and B. Q. , IQ-TREE: A fast and effective stochastic 646 algorithm for estimating maximum likelihood phylogenies, Mol Biol Evol, vol.32, pp.268-274, 2015.


H. Wickham, Elegant Graphics for Data Analysis, pp.33-74, 2016.

. York,