Skip to Main content Skip to Navigation
Journal articles

Protein crystallization promotes type 2 immunity and is reversible by antibody treatment

Abstract : Although spontaneous protein crystallization is a rare event in vivo, Charcot-Leyden crystals (CLCs) consisting of galectin-10 (Gal10) protein are frequently observed in eosinophilic diseases, such as asthma. We found that CLCs derived from patients showed crystal packing and Gal10 structure identical to those of Gal10 crystals grown in vitro. When administered to the airways, crystalline Gal10 stimulated innate and adaptive immunity and acted as a type 2 adjuvant. By contrast, a soluble Gal10 mutein was inert. Antibodies directed against key epitopes of the CLC crystallization interface dissolved preexisting CLCs in patient-derived mucus within hours and reversed crystal-driven inflammation, goblet-cell metaplasia, immunoglobulin E (IgE) synthesis, and bronchial hyperreactivity (BHR) in a humanized mouse model of asthma. Thus, protein crystals may promote hallmark features of asthma and are targetable by crystal-dissolving antibodies.
Document type :
Journal articles
Complete list of metadatas

https://hal-amu.archives-ouvertes.fr/hal-02542110
Contributor : Annick Prémilleux <>
Submitted on : Tuesday, April 14, 2020 - 2:32:44 PM
Last modification on : Friday, June 19, 2020 - 3:08:57 AM

Links full text

Identifiers

Collections

Citation

Emma Persson, Kenneth Verstraete, Ines Heyndrickx, Elien Gevaert, Helena Aegerter, et al.. Protein crystallization promotes type 2 immunity and is reversible by antibody treatment. Science, American Association for the Advancement of Science, 2019, 364 (6442), pp.eaaw4295. ⟨10.1126/science.aaw4295⟩. ⟨hal-02542110⟩

Share

Metrics

Record views

28