Adipose-Derived Stem Cells from Systemic Sclerosis Patients Maintain Pro-Angiogenic and Antifibrotic Paracrine Effects In Vitro - Archive ouverte HAL Access content directly
Journal Articles Journal of Clinical Medicine Year : 2019

Adipose-Derived Stem Cells from Systemic Sclerosis Patients Maintain Pro-Angiogenic and Antifibrotic Paracrine Effects In Vitro

(1, 2) , (2) , (3) , (2, 3) , (4, 5) , (5) , (2) , (6, 2) , (7, 2) , (8) , (2, 3) , (9, 2) , (2)
1
2
3
4
5
6
7
8
9
Maxime Abellan
  • Function : Author
  • PersonId : 1069764
Anaïs Lagrange
  • Function : Author

Abstract

Innovative therapies based on autologous adipose-derived stem/stromal cells (ASC) are currently being evaluated for treatment of systemic sclerosis (SSc). Although paracrine angiogenic and antifibrotic effects are considered the predominant mechanisms of ASC therapeutic potential, the impact of SSc on ASC paracrine functions remains controversial. In this study, phenotype, senescence, differentiation potential, and molecular profile were determined in ASC from SSc patients (SSc-ASC) (n = 7) and healthy donors (HD-ASC) (n = 7). ASC were co-cultured in indirect models with dermal fibroblasts (DF) from SSc patients or endothelial cells to assess their pro-angiogenic and antifibrotic paracrine effects. The angiogenic activity of endothelial cells was measured in vitro using tube formation and spheroid assays. DF collagen and alpha smooth muscle actin (αSMA) content were quantified after five days of co-culture with ASC. Differentiation capacity, senescence, and mRNA profiles did not differ significantly between SSc-ASC and HD-ASC. SSc-ASC retained the ability to stimulate angiogenesis through paracrine mechanisms; however, functional assays revealed reduced potential compared to HD-ASC. DF fibrosis markers were significantly decreased after co-culture with SSc-ASC. Together, these results indicate that SSc effects do not significantly compromise the angiogenic and the antifibrotic paracrine properties of ASC, thereby supporting further development of ASC-based autologous therapies for SSc treatment.
Fichier principal
Vignette du fichier
jcm-08-01979.pdf (2.18 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02592827 , version 1 (15-05-2020)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Mélanie Velier, Stephanie Simoncini, Maxime Abellan, Pauline Francois, Sandy Eap, et al.. Adipose-Derived Stem Cells from Systemic Sclerosis Patients Maintain Pro-Angiogenic and Antifibrotic Paracrine Effects In Vitro. Journal of Clinical Medicine, 2019, 8 (11), pp.1979. ⟨10.3390/jcm8111979⟩. ⟨hal-02592827⟩
43 View
55 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More