Skip to Main content Skip to Navigation
Journal articles

Molecular Detection of Spotted-Fever Group Rickettsiae in Ticks Collected from Domestic and Wild Animals in Corsica, France

Abstract : To obtain a better understanding of the current magnitude of tick-borne rickettsioses in Corsica, we used molecular methods to characterize the occurrence of Rickettsia spp. in ixodid ticks collected from domestic and wild animals. The presence of Rickettsia spp. was evaluated using real-time polymerase chain reaction targeting the gltA gene and by sequencing of gltA and ompA partial genes for species identification and phylogenetic analysis. Infection rates were calculated as the maximum-likelihood estimation (MLE) with 95% confidence intervals (CI). In total, 1117 ticks belonging to four genera (Rhipicephalus, Hyalomma, Ixodes, and Dermacentor) were collected from cattle, sheep, wild boars, and companion animals during July-August 2017 and July 2018-January 2019. Overall, Rickettsia DNA was detected in 208 of 349 pools of ticks (MLE = 25.6%, 95% CI: 22.6-28.8%). The molecular analysis revealed five different rickettsial species of the spotted-fever group (SFG). We highlighted the exclusive detection of Candidatus Ri. barbariae in R. bursa and of Ri. aeschlimanii in H. marginatum. Rickettsia slovaca was detected in D. marginatus collected from wild boars. This study provides the first evidence of the presence of Ri. monacensis in I. ricinus ticks isolated from a dog in Corsica. In conclusion, our data revealed wide dispersal of SFG Rickettsiae and their arthropod hosts in Corsica, highlighting the need for surveillance of the risk of infection for people living and/or working close to infected or infested animals.
Complete list of metadatas

https://hal-amu.archives-ouvertes.fr/hal-02617055
Contributor : Administrateur Hal Amu <>
Submitted on : Monday, May 25, 2020 - 9:47:15 AM
Last modification on : Wednesday, August 19, 2020 - 12:08:18 PM

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Links full text

Identifiers

Citation

Vincent Cicculli, Maestrini Oscar, François Casabianca, Natacha Villechenaud, Rémi Charrel, et al.. Molecular Detection of Spotted-Fever Group Rickettsiae in Ticks Collected from Domestic and Wild Animals in Corsica, France. Pathogens, Multidisciplinary Digital Publishing Institute, 2019, 8 (3), pp.138. ⟨10.3390/pathogens8030138⟩. ⟨hal-02617055⟩

Share

Metrics

Record views

53