Molecular Detection of Spotted-Fever Group Rickettsiae in Ticks Collected from Domestic and Wild Animals in Corsica, France

Vincent Cicculli (1) , Maestrini Oscar (2) , François Casabianca (2) , Natacha Villechenaud (1) , Rémi Charrel (3) , Xavier de Lamballerie (4) , Alessandra Falchi (5)

1 EA Bioscope Corse Méditerranée : Dynamique des infections virales en milieu insulaire
2 LRDE - Laboratoire de Recherches sur le Développement de l'Elevage
3 UVE - Unité des Virus Emergents
4 EPV - Emergence des Pathologies Virales
5 ESIM - Epidémiologie des maladies infectieuses et modélisation

To obtain a better understanding of the current magnitude of tick-borne rickettsioses in Corsica, we used molecular methods to characterize the occurrence of Rickettsia spp. in ixodid ticks collected from domestic and wild animals. The presence of Rickettsia spp. was evaluated using real-time polymerase chain reaction targeting the gltA gene and by sequencing of gltA and ompA partial genes for species identification and phylogenetic analysis. Infection rates were calculated as the maximum-likelihood estimation (MLE) with 95% confidence intervals (CI). In total, 1117 ticks belonging to four genera (Rhipicephalus, Hyalomma, Ixodes, and Dermacentor) were collected from cattle, sheep, wild boars, and companion animals during July-August 2017 and July 2018-January 2019. Overall, Rickettsia DNA was detected in 208 of 349 pools of ticks (MLE = 25.6%, 95% CI: 22.6-28.8%). The molecular analysis revealed five different rickettsial species of the spotted-fever group (SFG). We highlighted the exclusive detection of Candidatus Ri. barbariae in R. bursa and of Ri. aeschlimanii in H. marginatum. Rickettsia slovaca was detected in D. marginatus collected from wild boars. This study provides the first evidence of the presence of Ri. monacensis in I. ricinus ticks isolated from a dog in Corsica. In conclusion, our data revealed wide dispersal of SFG Rickettsiae and their arthropod hosts in Corsica, highlighting the need for surveillance of the risk of infection for people living and/or working close to infected or infested animals.

Mots clés: host; ticks; Rickettsia; Corsica; domestic animals; wild animals

Domaines: Sciences du Vivant [q-bio]; Maladies émergentes

Vincent Cicculli, Maestrini Oscar, François Casabianca, Natacha Villechenaud, Rémi Charrel, et al.. Molecular Detection of Spotted-Fever Group Rickettsiae in Ticks Collected from Domestic and Wild Animals in Corsica, France. Pathogens, 2019, 8 (3), pp.138. ⟨10.3390/pathogens8030138⟩. ⟨hal-02617055⟩