Brainstems were mostly cut coronally (40 µm sections), except for parvalbumin 662 labelling where they were cut sagitally (50 µm sections), using a cryostat. Fluorescence 663 immunohistochemistry was performed as previously described, Primary antibodies used were rabbit anti-TH, pp.5000-665, 2010. ,
, AB13970), mouse anti-parvalbumin 667 (1:10,000, Merck-Millipore Bioscience Research Reagents, p.668
, Rabbit anti-NK1R (1:5000, Merck-Sigma, p.632496
, Goat anti-ChAT (1:1000; Merck-Millipore Bioscience Research Reagents, p.671
The secondary antibodies used were Cy3-conjugated donkey anti-rabbit 672 (1:500, Jackson ImmunoResearch Laboratories, AlexaFluor-674 488 donkey anti-rabbit, p.500 ,
, AlexaFluor-488 donkey anti-mouse (1:500
USA) and AlexaFluor-488 donkey anti-chicken (1:500, p.677 ,
,
, TH-positive C1 neurons in the rostral ventrolateral medulla (Di and Dii), and (E) ChAT-981 positive neurons in the compact formation of the nucleus ambiguus (AmbC) (Ei). Scale bars 982 are 500 µm, p.100
, Hz preBötC photoinhibition is correlated to RespSNA AUC. Two-way repeated 1084 measures ANOVA followed with post hoc Holm-Sidak multiple-comparison test, as shown 1085 on the associated source data and detailed statistics
*** p < 0.001, 1086 photoinhibition vs. control and recovery conditions ; ### p < 0.001, between photostimulation 1087 frequencies. 1088 respiratory cycle as observed in PNA burst-triggered averages of HR ,
RespSNA peaks at the transition between inspiration and post-1103 inspiration, and the RespSNA burst is mainly composed of an inspiratory component, and a 1104 smaller post-inspiratory component. Traube-Hering waves, induced by RespSNA, peak 1105 during post-inspiration and therefore with a delay compared to RespSNA, due to the slow 1106 vascular contraction dynamics. The duration of the respiratory phases was impacted by 1107 preBötC photoinhibition ,
, Hering waves, yet their timing relative to the respiratory cycle remained unaltered. One-way 1109 repeated measures ANOVA followed with post hoc Holm-Sidak multiple-comparison test or
, Friedman repeated measures analysis of variance on ranks followed with pairwise multiple 1111 comparison Tukey test, as shown on the associated source data and detailed statistics
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