A Chemogenetic Tool that Enables Functional Neural Circuit Analysis

Hoai Buu Ngo (1) , Mariana R Melo (2) , Ross a D Bathgate (1, 2) , Sharon Layfield (1) , Angela Connelly (2) , Jaspreet Bassi (2) , Lin Xie (2) , Clément Menuet (3) , Stuart J Mcdougall (1) , Ross A.D. Bathgate (2) , Andrew M Allen (1, 2)

1 Howard Florey Institute
2 University of Melbourne
3 INMED - INSERM U1249 - Institut de Neurobiologie de la Méditerranée [Aix-Marseille Université]

Graphical Abstract Highlights d Oxytocin is replaced in its precursor with the insect peptide allatostatin (Ast) d Viral expression induces activity-dependent, synaptic release of bioactive Ast d Ast is expressed in vagal afferents and Ast receptor in Phox2 NTS neurons d This induces a long-term reduction in baroreceptor sensitivity and bodyweight gain

Keywords: allatostatin; chemogenetics; viral transduction; viscerosensory system; baroreceptor sensitivity; vagus; nucleus of the solitary tract; bodyweight

Domains: Life Sciences [q-bio]; Neurons and Cognition [q-bio.NC]

Hoai Buu Ngo, Mariana R Melo, Ross a D Bathgate, Sharon Layfield, Angela Connelly, et al.. A Chemogenetic Tool that Enables Functional Neural Circuit Analysis. Cell Reports, In press, 32 (11), pp.108139. ⟨10.1016/j.celrep.2020.108139⟩. ⟨hal-02963987⟩