Skip to Main content Skip to Navigation
Journal articles

Establishing rod shape from spherical, peptidoglycan-deficient bacterial spores

Abstract : Chemical-induced spores of the Gram-negative bacterium Myxococcus xanthus are peptidoglycan (PG)-deficient. It is unclear how these spherical spores germinate into rod-shaped, walled cells without preexisting PG templates. We found that germinating spores first synthesize PG randomly on spherical surfaces. MglB, a GTPase-activating protein, forms a cluster that responds to the status of PG growth and stabilizes at one future cell pole. Following MglB, the Ras family GTPase MglA localizes to the second pole. MglA directs molecular motors to transport the bacterial actin homolog MreB and the Rod PG synthesis complexes away from poles. The Rod system establishes rod shape de novo by elongating PG at nonpolar regions. Thus, similar to eukaryotic cells, the interactions between GTPase, cytoskeletons, and molecular motors initiate spontaneous polarization in bacteria.
Document type :
Journal articles
Complete list of metadatas

Cited literature [51 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-02995861
Contributor : Delphine Leroi <>
Submitted on : Tuesday, November 10, 2020 - 3:16:20 PM
Last modification on : Wednesday, November 18, 2020 - 9:43:21 AM

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Huan Zhang, Garrett Mulholland, Sofiene Seef, Shiwei Zhu, Jun Liu, et al.. Establishing rod shape from spherical, peptidoglycan-deficient bacterial spores. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2020, 117 (25), pp.14444-14452. ⟨10.1073/pnas.2001384117⟩. ⟨hal-02995861⟩

Share

Metrics

Record views

29

Files downloads

33