Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth

Jan Krivanek (1, 2) , Ruslan Soldatov (3) , Maria Eleni Kastriti (1, 4) , Tatiana Chontorotzea (1) , Anna Nele Herdina (4) , Julian Petersen (1, 4) , Bara Szarowska (1) , Marie Landova (5) , Veronika Kovar Matejova (2) , Lydie Izakovicova Holla (2) , Ulrike Kuchler (1) , Ivana Vidovic Zdrilic (6) , Anushree Vijaykumar (6) , Anamaria Balic (7) , Pauline Marangoni (8) , Ophir Klein (8) , Vitor Neves (9) , Val Yianni (9) , Paul Sharpe (9) , Tibor Harkany (1, 4) , Brian Metscher (10) , Marc Bajenoff (11) , Mina Mina (6) , Kaj Fried (4) , Peter Kharchenko (3) , Igor Adameyko (1, 4)

1 Medizinische Universität Wien = Medical University of Vienna
2 MUNI - Masaryk University [Brno]
3 HMS - Harvard Medical School [Boston]
4 Karolinska Institutet [Stockholm]
5 IAPG / CAS - Institute of Animal Physiology and Genetics of the Czech Academy of Sciences
6 University of Connecticut Health Center [Farmington]
7 Helsingin yliopisto = Helsingfors universitet = University of Helsinki
8 UC - University of California
9 King‘s College London
10 University of Vienna [Vienna]
11 CIML - Centre d'Immunologie de Marseille - Luminy

Understanding cell types and mechanisms of dental growth is essential for reconstruction and engineering of teeth. Therefore, we investigated cellular composition of growing and non-growing mouse and human teeth. As a result, we report an unappreciated cellular complexity of the continuously-growing mouse incisor, which suggests a coherent model of cell dynamics enabling unarrested growth. This model relies on spatially-restricted stem, progenitor and differentiated populations in the epithelial and mesenchymal compartments underlying the coordinated expansion of two major branches of pulpal cells and diverse epithelial subtypes. Further comparisons of human and mouse teeth yield both parallelisms and differences in tissue heterogeneity and highlight the specifics behind growing and non-growing modes. Despite being similar at a coarse level, mouse and human teeth reveal molecular differences and species-specific cell subtypes suggesting possible evolutionary divergence. Overall, here we provide an atlas of human and mouse teeth with a focus on growth and differentiation.

Mots clés: Mesenchymal stem cells; Organogenesis; Stem-cell niche

Domaines: Sciences du Vivant [q-bio]

Jan Krivanek, Ruslan Soldatov, Maria Eleni Kastriti, Tatiana Chontorotzea, Anna Nele Herdina, et al.. Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth. Nature Communications, 2020, 11 (1), ⟨10.1038/s41467-020-18512-7⟩. ⟨hal-02998769⟩