Clicked Bifunctional Dendrimeric and Cyclopeptidic Addressable Redox Scaffolds for the Functionalization of Carbon Nanotubes with Redox Molecules and Enzymes

Solène Gentil (1, 2) , Carlo Pifferi (1) , Pierre Rousselot-Pailley (3) , Thierry Tron (3) , Olivier Renaudet (1) , Alan Le Goff (1)

1 DCM - Département de Chimie Moléculaire
2 SolHyCat - Solar fuels, hydrogen and catalysis
3 ISM2 - Institut des Sciences Moléculaires de Marseille

Carbon nanotube electrodes were modified with ferrocene and laccase using two different click reactions strategies and taking advantage of bifunctional dendrimers and cyclopeptides. Using diazonium functionalization and the efficiency of oxime ligation, the combination of both multiwalled carbon nanotube surfaces and modified dendrimers or cyclopeptides allows the access to a high surface coverage of ferrocene in the order of 50 nmol cm–2, a 50-fold increase compared to a classic click reaction without oxime ligation of these highly branched macromolecules. Furthermore, this original immobilization strategy allows the immobilization of mono- and bi-functionalized active multicopper enzymes, laccases, via copper(I)-catalyzed azide–alkyne cycloaddition. Electrochemical studies underline the high efficiency of the oxime-ligated dendrimers or cyclopeptides for the immobilization of redox entities on surfaces while being detrimental to electron tunneling with enzyme active sites despite controlled orientation.

Domaines: Matériaux

Solène Gentil, Carlo Pifferi, Pierre Rousselot-Pailley, Thierry Tron, Olivier Renaudet, et al.. Clicked Bifunctional Dendrimeric and Cyclopeptidic Addressable Redox Scaffolds for the Functionalization of Carbon Nanotubes with Redox Molecules and Enzymes. Langmuir, 2021, 37 (3), pp.1001-1011. ⟨10.1021/acs.langmuir.0c02095⟩. ⟨hal-03132807⟩