Elemental quantification and analysis of structural abnormalities in neurons from Parkinson's-diseased brains by X-ray fluorescence microscopy and diffraction

Karina Joppe (1) , Jan-David Nicolas (2) , Tilman Grünewald (3, 4, 5) , Marina Eckermann (2) , Tim Salditt (2) , Paul Lingor (1)

1 UMG - University Medical Center Göttingen
2 Georg-August-University = Georg-August-Universität Göttingen
3 COMiX - Coherent Optical Microscopy and X-rays
4 ESRF - European Synchrotron Radiation Facility
5 FRESNEL - Institut FRESNEL

In this work we use scanning X-ray microscopy to study the structure and elemental composition of neuromelanin-positive neurons in substantia nigra tissue of Parkinson patients (PD) and controls. A total of 53 neurons were analyzed with X-ray fluorescence (XRF) and diffraction using sub-mu m-focused synchrotron radiation. A statistical evaluation identified copper as the most group-discriminating element and indicated that interindividual and intraindividual variations are of great relevance in tissue measurements of diseased patients and prevent from automated group clustering. XRF analyses of two Lewy bodies (LBs) highlight a heterogeneity in elemental distributions in these LBs, whereas an innovative X-ray diffraction-based method approach was used to reveal beta-sheet-rich crystalline structures in LBs. Overall, sub-mu m-focus X-ray microscopy highlighted the elemental heterogeneity in PD pathology. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

Keywords: ALPHA-SYNUCLEIN; SUBSTANTIA-NIGRA; LEWY BODIES; IRON; COPPER; METALS; FIBRILLATION; BINDING; TISSUES; CELLS

Domains: Life Sciences [q-bio]

Karina Joppe, Jan-David Nicolas, Tilman Grünewald, Marina Eckermann, Tim Salditt, et al.. Elemental quantification and analysis of structural abnormalities in neurons from Parkinson's-diseased brains by X-ray fluorescence microscopy and diffraction. Biomedical optics express, 2020, 11 (7), pp.3423-3443. ⟨10.1364/BOE.389408⟩. ⟨hal-03139973⟩