Skip to Main content Skip to Navigation
Journal articles

Comparison of Three Skin Sampling Methods and Two Media for Culturing Malassezia Yeast

Abstract : Malassezia is a lipid-dependent commensal yeast of the human skin. The different culture media and skin sampling methods used to grow these fastidious yeasts are a source of heterogeneity in culture-based epidemiological study results. This study aimed to compare the performances of three methods of skin sampling, and two culture media for the detection of Malassezia yeasts by culture from the human skin. Three skin sampling methods, namely sterile gauze, dry swab, and TranswabTM with transport medium, were applied on 10 healthy volunteers at 5 distinct body sites. Each sample was further inoculated onto either the novel FastFung medium or the reference Dixon agar for the detection of Malassezia spp. by culture. At least one colony of Malassezia spp. grew on 93/300 (31%) of the cultures, corresponding to 150 samplings. The positive culture rate was 67%, 18%, and 15% (P < 10−3), for samples collected with sterile gauze, TranswabTM, and dry swab, respectively. The positive culture rate was 62% and 38% (P < 0.003) by using the FastFung and the Dixon media, respectively. Our results showed that sterile gauze rubbing skin sampling followed by inoculation on FastFung medium should be implemented in the routine clinical laboratory procedure for Malassezia spp. cultivation.
Complete list of metadatas

https://hal-amu.archives-ouvertes.fr/hal-03141598
Contributor : Isabelle Combe <>
Submitted on : Monday, February 15, 2021 - 1:44:41 PM
Last modification on : Tuesday, February 16, 2021 - 3:30:04 AM

File

jof-06-00350.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Abdourahim Abdillah, Saber Khelaifia, Didier Raoult, Fadi Bittar, Stephane Ranque. Comparison of Three Skin Sampling Methods and Two Media for Culturing Malassezia Yeast. Journal of Fungi, MDPI, 2020, 6 (4), pp.350. ⟨10.3390/jof6040350⟩. ⟨hal-03141598⟩

Share

Metrics

Record views

36

Files downloads

31