Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells

Mégane Delourme (1) , Natacha Broucqsault (1) , Kilian Mazaleyrat (1) , Frédérique Magdinier (1)

1 MMG - Marseille medical genetics - Centre de génétique médicale de Marseille

Only a limited number of large-scale protocols describe the production of mature skeletal muscle fibers from human induced pluripotent stem cells (hiPSCs). Here we describe a novel procedure for simultaneous differentiation of hiPSC into muscle cells and motor neurons, that generates innervated and contractile multinucleated skeletal muscle fibers with sarcomeric organization. Our protocol permits the production of expandable skeletal muscle progenitor cells and mature skeletal muscle fibers that can be used for the exploration of skeletal muscle differentiation for basic research, disease modeling, and drug discovery.

Keywords: Stem cells; Myoblasts; Myotubes; Motor neurons; Muscle fiber; Contraction; Sarcomere; Neuromuscular junction; Neuromuscular disease modeling; Stem cells

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Biochemistry, Molecular Biology

Mégane Delourme, Natacha Broucqsault, Kilian Mazaleyrat, Frédérique Magdinier. Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells. Methods in Molecular Biology, 2020, ⟨10.1007/7651_2020_334⟩. ⟨hal-03142891⟩