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Contribution of VitaPCR SARS-CoV-2 to the emergency diagnosis of COVID-19

Abstract : Background: With the persistent COVID-19 pandemic, there is an urgent need to use rapid and reliable diagnostic tools for highly urgent cases. Antigen tests are disappointing with their lack of sensitivity. Among molecular tools allowing a diagnosis in less than an hour, only one, the Cepheid Xpert Xpress SARS-CoV-2 assay, has exhibited a good sensitivity. However, we are also facing a global shortage of reagents and kits. Thus, it is imperative to evaluate other point-of-care molecular tests. Methods: We evaluated the VitaPCR (TM) RT-PCR assay, whose sample analysis time is of approximately 20 min, in nasopharyngeal secretions from 534 patients presenting to our Institute, for the diagnosis of COVID-19, and compared it to our routine RT-PCR assay. We also compared the two assays with tenfold dilutions of a SARS-CoV2 strain. Results: Compared to our routine RT-PCR and the previous diagnosis of COVID-19, the sensitivity, specificity, positive and negative predictive values of VitaPCRTM can be evaluated to be 99.3 % (155/156), 94.7 % (358/378), 88.6 % (155/175) and 99.7 % (358/359), respectively. Tenfold dilutions of a SARS-CoV-2 strain show that the VitaPCR (TM) was more sensitive that our routine RT-PCR assay. Conclusion: The VitaPCR (TM) SARS-CoV-2 is an accurate rapid test, suitable for clinical practice that can be performed as part of a point-of-care testing, for the rapid diagnosis of COVID-19.
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Contributor : Isabelle Combe <>
Submitted on : Monday, February 22, 2021 - 8:01:13 PM
Last modification on : Wednesday, March 17, 2021 - 9:26:03 AM

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Pierre-Edouard Fournier, Christine Zandotti, Laetitia Ninove, Elsa Prudent, Philippe Colson, et al.. Contribution of VitaPCR SARS-CoV-2 to the emergency diagnosis of COVID-19. JOURNAL OF CLINICAL VIROLOGY, 2020, 133, ⟨10.1016/j.jcv.2020.104682⟩. ⟨hal-03149234⟩



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