HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Contribution of VitaPCR SARS-CoV-2 to the emergency diagnosis of COVID-19

Abstract : Background: With the persistent COVID-19 pandemic, there is an urgent need to use rapid and reliable diagnostic tools for highly urgent cases. Antigen tests are disappointing with their lack of sensitivity. Among molecular tools allowing a diagnosis in less than an hour, only one, the Cepheid Xpert Xpress SARS-CoV-2 assay, has exhibited a good sensitivity. However, we are also facing a global shortage of reagents and kits. Thus, it is imperative to evaluate other point-of-care molecular tests. Methods: We evaluated the VitaPCR (TM) RT-PCR assay, whose sample analysis time is of approximately 20 min, in nasopharyngeal secretions from 534 patients presenting to our Institute, for the diagnosis of COVID-19, and compared it to our routine RT-PCR assay. We also compared the two assays with tenfold dilutions of a SARS-CoV2 strain. Results: Compared to our routine RT-PCR and the previous diagnosis of COVID-19, the sensitivity, specificity, positive and negative predictive values of VitaPCRTM can be evaluated to be 99.3 % (155/156), 94.7 % (358/378), 88.6 % (155/175) and 99.7 % (358/359), respectively. Tenfold dilutions of a SARS-CoV-2 strain show that the VitaPCR (TM) was more sensitive that our routine RT-PCR assay. Conclusion: The VitaPCR (TM) SARS-CoV-2 is an accurate rapid test, suitable for clinical practice that can be performed as part of a point-of-care testing, for the rapid diagnosis of COVID-19.
Document type :
Journal articles
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03149234
Contributor : Isabelle Combe Connect in order to contact the contributor
Submitted on : Monday, February 22, 2021 - 8:01:13 PM
Last modification on : Friday, February 4, 2022 - 3:26:16 AM

Links full text

Identifiers

Collections

Citation

Pierre-Edouard Fournier, Christine Zandotti, Laetitia Ninove, Elsa Prudent, Philippe Colson, et al.. Contribution of VitaPCR SARS-CoV-2 to the emergency diagnosis of COVID-19. JOURNAL OF CLINICAL VIROLOGY, 2020, 133, ⟨10.1016/j.jcv.2020.104682⟩. ⟨hal-03149234⟩

Share

Metrics

Record views

17