Skip to Main content Skip to Navigation
Journal articles

Hymenopteran Parasitoids of Hard Ticks in Western Africa and the Russian Far East

Abstract : Some parasitoids of the genus Ixodiphagus (Hymenoptera, Chalcidoidea: Encyrtidae) are well-known natural enemies of ticks. In this study, we investigate the occurrence of parasitoid wasps in adult hard ticks from Western Africa (Cote d'Ivoire and Senegal) and Far Eastern Europe (Russia) using molecular methods. The morphological identification allowed the classification of 785 collected specimens of six species of ticks: Rhipicephalus (Boophilus) microplus (41%), Ixodes persulcatus (33%), Dermacentor silvarum (11%), Haemaphysalis concinna (7%), Amblyomma variegatum (5%), and Haemaphysalis japonica (3%). The newly developed MALDI-TOF MS protocol identified tick species in spite of their different storage (dried or in 70% ethanol) conditions for a long period. Molecular screening of ticks by a new standard PCR system developed in this study revealed the presence of parasitoid wasp DNA in 3% (28/785) of analyzed ticks. Ixodiphagus hookeri was detected in 86% (24/28) of infested ticks, including 13 I. persulcatus, 9 R (B) microplus, and one H. concinna and D. silvarum. While an unidentified parasitoid wasp species from the subfamily Aphidiinae and Braconidae family was detected in the remaining 14% (4/28) infested ticks. These infested ticks were identified as I. persulcatus. Our findings highlight the need for further studies to clarify the species diversity of parasitoid infesting ticks by combining molecular and morphological features. The novel molecular and MALDI-TOF MS protocols could be effective tools for the surveillance and characterization of these potential bio-control agents of ticks.
Document type :
Journal articles
Complete list of metadata
Contributor : Isabelle COMBE Connect in order to contact the contributor
Submitted on : Monday, February 22, 2021 - 8:01:23 PM
Last modification on : Friday, February 4, 2022 - 3:26:19 AM

Links full text




Mapenda Gaye, Nadia Amanzougaghene, younes Laidoudi, El Hadji Amadou Niang, Zuzana Sekeyova, et al.. Hymenopteran Parasitoids of Hard Ticks in Western Africa and the Russian Far East. Microorganisms, MDPI, 2020, 8 (12), ⟨10.3390/microorganisms8121992⟩. ⟨hal-03149238⟩



Record views