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Investigation of an XDR-Acinetobacter baumannii ST2 outbreak in an intensive care unit of a Lebanese tertiary care hospital

Marwan Osman 1 Fatima B. Halimeh 1, 2 Rayane Rafei 1 Hassan Mallat 1 Jad El Tom Elie B. Raad Seydina M. Diene 2 Sabah Jamal 1 Ahmad Al Atrouni Fouad Dabboussi 1 Walid Moudani 3 Jean-Yves Madec 4 Estelle Saras 5 Agnese Lupo 6 Marisa Haenni 4 Jean-Marc Rolain 2, 7 Monzer Hamze 8
1 LMSE - Laboratoire Microbiologie Santé et Environnement [Tripoli, Liban]
2 MEPHI - Microbes évolution phylogénie et infections
3 LU - Lebanese University [Beirut]
4 Unité Antibiorésistance et Virulence Bactériennes
Laboratoire de Lyon [ANSES]
5 Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes
6 Unité Antibiorésistance et Virulence Bactériennes
7 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
8 Université Libanaise
Abstract : Aim: We sought to investigate the genetic epidemiological relatedness of carbapenem-resistant Acinetobacter baumannii (CRAB) strains of a suspected outbreak in a Lebanese tertiary care hospital to implement necessary infection prevention and control measures. Methods: Twenty-eight nonduplicate CRAB isolates detected among hospitalized patients between January 2016 and July 2017 were studied by real-time polymerase chain reaction (PCR), pulsed-field gel electrophoresis and multilocus sequence typing analyses. Results: Twenty-seven isolates harbored bla(OXA-23). of which one also carried bla(NDM-1). The isolates distributed temporally in two presumably episodes were stratified by pulsed-field gel electrophoresis into many clusters. Although several clones have become endemic in the hospital, we have rapidly implemented appropriate infection prevention and control measures, achieving full eradication from August 2017 to November 2019. Conclusion: We have successfully investigated and controlled a polyclonal outbreak of OXA-23 producing ST2 CRAB.
Keywords : Molecular typing Intensive Care Units Acinetobacter baumannii Epidemiology Lebanon
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https://hal-amu.archives-ouvertes.fr/hal-03149247
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Submitted on : Monday, February 22, 2021 - 8:01:43 PM
Last modification on : Wednesday, November 3, 2021 - 5:17:21 AM

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Marwan Osman, Fatima B. Halimeh, Rayane Rafei, Hassan Mallat, Jad El Tom, et al.. Investigation of an XDR-Acinetobacter baumannii ST2 outbreak in an intensive care unit of a Lebanese tertiary care hospital. Future Microbiology, 2020, 15 (16), pp.1535-1542. ⟨10.2217/fmb-2020-0079⟩. ⟨hal-03149247⟩

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