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Screening anti-infectious molecules against Mycobacterium ulcerans: A step towards decontaminating environmental specimens

Abstract : Mycobacterium ulcerans, a non-tuberculous mycobacterium responsible for Buruli ulcer, resides in poorly defined environmental niches in the vicinity of stagnant water. Very few isolates have been confirmed. With a view to culturingM.ulceransfrom such contaminated environmental specimens, we tested thein vitrosusceptibility of theM.ulceransCU001 strain co-cultivated with XTC cells to anti-infectious molecules registered in the French pharmacopoeia. We used a standardised concentration to identify molecules that were inactive againstM.ulceransand which could be incorporated into a decontaminating solution. Of 116 tested molecules, 64 (55.1%) molecules were ineffective against M. ulcerans CU001. These included 34 (29.3%) antibiotics, 14 (12%) antivirals, eight (6.8%) antiparasitics, and eight (6.8%) antifungals. This left 52 molecules which were active againstM.ulceransCU001. Three of the inactive antimicrobial molecules (oxytetracycline, polymyxin E and voriconazole) were then selected to prepare a decontamination solution which was shown to respectM.ulceransCU001 viability. These three antimicrobials could be incorporated into a decontamination solution to potentially isolate and cultureM.ulceransfrom environmental samples.
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Submitted on : Tuesday, February 23, 2021 - 11:54:12 AM
Last modification on : Wednesday, March 17, 2021 - 9:26:04 AM

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Nassim Hammoudi, Romain Verdot, Jean Delorme, Amar Bouam, Michel Drancourt. Screening anti-infectious molecules against Mycobacterium ulcerans: A step towards decontaminating environmental specimens. PLoS ONE, Public Library of Science, 2020, 15 (8), ⟨10.1371/journal.pone.0231685⟩. ⟨hal-03149728⟩

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