Screening anti-infectious molecules against Mycobacterium ulcerans: A step towards decontaminating environmental specimens
Résumé
Mycobacterium ulcerans, a non-tuberculous mycobacterium responsible for Buruli ulcer, resides in poorly defined environmental niches in the vicinity of stagnant water. Very few isolates have been confirmed. With a view to culturingM.ulceransfrom such contaminated environmental specimens, we tested thein vitrosusceptibility of theM.ulceransCU001 strain co-cultivated with XTC cells to anti-infectious molecules registered in the French pharmacopoeia. We used a standardised concentration to identify molecules that were inactive againstM.ulceransand which could be incorporated into a decontaminating solution. Of 116 tested molecules, 64 (55.1%) molecules were ineffective against M. ulcerans CU001. These included 34 (29.3%) antibiotics, 14 (12%) antivirals, eight (6.8%) antiparasitics, and eight (6.8%) antifungals. This left 52 molecules which were active againstM.ulceransCU001. Three of the inactive antimicrobial molecules (oxytetracycline, polymyxin E and voriconazole) were then selected to prepare a decontamination solution which was shown to respectM.ulceransCU001 viability. These three antimicrobials could be incorporated into a decontamination solution to potentially isolate and cultureM.ulceransfrom environmental samples.