Skip to Main content Skip to Navigation
Journal articles

Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts

Abstract : In French Guiana, the malaria, a parasitic infection transmitted by Anopheline mosquitoes, remains a disease of public health importance. To prevent malaria transmission, the main effective way remains Anopheles control. For an effective control, accurate Anopheles species identification is indispensable to distinguish malaria vectors from non-vectors. Although, morphological and molecular methods are largely used, an innovative tool, based on protein pattern comparisons, the Matrix Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) profiling, emerged this last decade for arthropod identification. However, the limited mosquito fauna diversity of reference MS spectra remains one of the main drawback for its large usage. The aim of the present study was then to create and to share reference MS spectra for the identification of French Guiana Anopheline species. A total of eight distinct Anopheles species, among which four are malaria vectors, were collected in 6 areas. To improve Anopheles identification, two body parts, legs and thoraxes, were independently submitted to MS for the creation of respective reference MS spectra database (DB). This study underlined that double checking by MS enhanced the Anopheles identification confidence and rate of reliable classification. The sharing of this reference MS spectra DB should make easier Anopheles species monitoring in endemic malaria area to help malaria vector control or elimination programs.
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03160937
Contributor : Isabelle Combe Connect in order to contact the contributor
Submitted on : Thursday, June 10, 2021 - 11:31:48 AM
Last modification on : Thursday, November 18, 2021 - 5:04:04 PM
Long-term archiving on: : Saturday, September 11, 2021 - 6:31:22 PM

File

pone.0234098.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Sébastien Briolant, Monique Melo Costa, Christophe Nguyen, Isabelle Dusfour, Vincent Pommier de Santi, et al.. Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts. PLoS ONE, Public Library of Science, 2020, 15 (8), pp.e0234098. ⟨10.1371/journal.pone.0234098⟩. ⟨hal-03160937⟩

Share

Metrics

Record views

119

Files downloads

54