fosM , a New Family of Fosfomycin Resistance Genes Identified in Bacterial Species Isolated from Human Microbiota

Sami Khabthani (1, 2) , Mouna Hamel (1, 2) , Sophie Alexandra Baron (1, 2) , Seydina Diene (1, 2) , Jean-Marc Rolain (1, 2) , Vicky Merhej (1, 2)

1 MEPHI - Microbes évolution phylogénie et infections
2 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection

ABSTRACT Fosfomycin is a decades-old antibiotic, currently reused because of its activity against multidrug-resistant bacteria. Here, we used a combined in vitro/in silico approach to search for fosfomycin resistance determinants in 25 new bacterial species isolated from the human microbiota. Putative resistance genes were cloned into a susceptible Escherichia coli strain. MIC values increased from 1 μg/ml to 1,024 μg/ml. Here, we report a new family of potential chromosomal fosfomycin resistance genes, named fosM .

Mots clés: FosM; bacillithiol transferase; fosfomycin resistance; genomes; human microbiota; phylogenetics

Domaines: Bactériologie; Virologie; Parasitologie; Cardiologie et système cardiovasculaire; Maladies émergentes; Maladies infectieuses

Sami Khabthani, Mouna Hamel, Sophie Alexandra Baron, Seydina Diene, Jean-Marc Rolain, et al.. fosM , a New Family of Fosfomycin Resistance Genes Identified in Bacterial Species Isolated from Human Microbiota. Antimicrobial Agents and Chemotherapy, 2021, 65 (2), ⟨10.1128/AAC.01712-20⟩. ⟨hal-03216141⟩