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The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux

Abstract : With the spreading of antibiotic resistance, the translocation of antibiotics through bacterial envelopes is crucial for their antibacterial activity. In Gram-negative bacteria, the interplay between membrane permeability and drug efflux pumps must be investigated as a whole. Here, we quantified the intracellular accumulation of a series of fluoroquinolones in population and in individual cells of Escherichia coli according to the expression of the AcrB efflux transporter. Computational results supported the accumulation levels measured experimentally and highlighted how fluoroquinolones side chains interact with specific residues of the distal pocket of the AcrB tight monomer during recognition and binding steps.
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https://hal-amu.archives-ouvertes.fr/hal-03220501
Contributor : Emmanuelle Rosa <>
Submitted on : Friday, May 7, 2021 - 11:48:51 AM
Last modification on : Thursday, July 1, 2021 - 4:46:28 PM

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Julia Vergalli, Alessio Atzori, Jelena Pajovic, Estelle Dumont, Giuliano Malloci, et al.. The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux. Communications Biology, Nature Publishing Group, 2020, 3 (1), ⟨10.1038/s42003-020-0929-x⟩. ⟨hal-03220501⟩

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