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Involvement of the twin-arginine translocation system in protein secretion via the type II pathway

Abstract : The general secretory pathway (GSP) is a two-step process for the secretion of proteins by Gram-negative bacteria. The translocation across the outer membrane is carried out by the type II system, which involves machinery called the secreton. This step is considered to be an extension of the general export pathway, i.e. the export of proteins across the inner membrane by the Sec machinery. Here, we demonstrate that two substrates for the Pseudomonas aeruginosa secreton, both phospholipases, use the twinarginine translocation (Tat) system, instead of the Sec system, for the ®rst step of translocation across the inner membrane. These results challenge the previous vision of the GSP and suggest for the ®rst time a mosaic model in which both the Sec and the Tat systems feed substrates into the secreton. Moreover, since P.aeruginosa phospholipases are secreted virulence factors, the Tat system appears to be a novel determinant of bacterial virulence.
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https://hal-amu.archives-ouvertes.fr/hal-03269180
Contributor : Berengere Ize <>
Submitted on : Wednesday, June 23, 2021 - 6:49:53 PM
Last modification on : Monday, July 12, 2021 - 9:39:14 AM
Long-term archiving on: : Friday, September 24, 2021 - 7:03:28 PM

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Romé Voulhoux, Genevieve Ball, Bérengère Ize, Michael Vasil, Andrée Lazdunski, et al.. Involvement of the twin-arginine translocation system in protein secretion via the type II pathway. EMBO Journal, EMBO Press, 2001, 20 (23), pp.6735-6741. ⟨10.1093/emboj/20.23.6735⟩. ⟨hal-03269180⟩

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