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Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system

Abstract : PorM is a membrane protein involved in the assembly of the type IX secretion system (T9SS) from Porphyromonas gingivalis , a major bacterial pathogen responsible for periodontal disease in humans. The periplasmic domain of PorM was overexpressed in Escherichia coli and purified. A fragment of the purified protein was obtained by limited proteolysis. Crystals of this fragment belonged to the tetragonal space group P 4 3 2 1 2. Native and MAD data sets were recorded to 2.85 and 3.1 Å resolution, respectively, using synchrotron radiation.
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https://hal-amu.archives-ouvertes.fr/hal-03274774
Contributor : Eric Cascales <>
Submitted on : Wednesday, June 30, 2021 - 2:18:26 PM
Last modification on : Monday, July 12, 2021 - 9:39:12 AM

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Julien Stathopulos, Christian Cambillau, E. Cascales, Alain Roussel, Philippe Leone. Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system. Acta crystallographica. Section F, Structural biology communications, John Wiley & Sons Ltd,, 2015, 71 (1), pp.71-74. ⟨10.1107/S2053230X1402559X⟩. ⟨hal-03274774⟩

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