Skip to Main content Skip to Navigation
Journal articles

The C‐tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci‐1 Type VI secretion system, is inserted by YidC

Abstract : Type VI secretion systems (T6SS) are macromolecular complexes present in Gram-negative bacteria. T6SS are structurally similar to the bacteriophage cell-puncturing device and have been shown to mediate bacteria–host or bacteria–bacteria interactions. T6SS assemble from 13 to 20 proteins. In enteroaggregative Escherichia coli (EAEC), one of the subassemblies is composed of four proteins that form a trans-envelope complex: the TssJ outer membrane lipoprotein, the peptidoglycan-anchored inner membrane TagL protein, and two putative inner membrane proteins, TssL and TssM. In this study, we characterized the TssL protein of the EAEC Sci-1 T6SS in terms of localization, topology, and function. TssL is a critical component of the T6SS, anchored to the inner membrane through a single transmembrane segment located at the extreme C-terminus of the protein. We further show that this transmembrane segment is essential for the function of the protein and its proper insertion in the inner membrane is dependent upon YidC and modulated by the Hsp70 homologue DnaK.
Document type :
Journal articles
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03274798
Contributor : Eric Cascales <>
Submitted on : Monday, July 12, 2021 - 11:10:17 AM
Last modification on : Monday, July 19, 2021 - 1:16:53 PM

File

mbo3.9.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Collections

Citation

Marie-Stéphanie Aschtgen, Abdelrahim Zoued, Roland Lloubès, Laure Journet, E. Cascales. The C‐tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci‐1 Type VI secretion system, is inserted by YidC. MicrobiologyOpen, Wiley, 2012, 1 (1), pp.71-82. ⟨10.1002/mbo3.9⟩. ⟨hal-03274798⟩

Share

Metrics

Record views

32

Files downloads

9